Development of a new method for detection of platelet factor 3 like activity.

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dc.contributor.authorOpartkiattikul, Nen_US
dc.contributor.authorFunahara, Yen_US
dc.contributor.authorHijikata-Okunomiya, Aen_US
dc.contributor.authorYamaguchi, Nen_US
dc.contributor.authorTalalak, Pen_US
dc.date.accessioned2009-05-27T16:10:27Z
dc.date.available2009-05-27T16:10:27Z
dc.date.issued1992-03-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractWe asked the question, "Can thalassemic erythrocytes play some role in alteration of the hemostatic system?", because clinical examination of thalassemic patients shows symptoms and signs related to alterations in hemostatic and circulatory systems, and thalassemic erythrocytes are different from normal erythrocytes. We obtained one of the answers to the question: The erythrocytes of postsplenectomized patients of beta-thalassemia/HbE disease could stimulate their own platelets to aggregate spontaneously. To know the role of erythrocytes in platelet aggregation, we wanted to examine the effect of thalassemic erythrocytes on the coagulation system by focusing of PF3-like activity of erythrocytes, because PF3-like activity of the ghosts of erythrocytes had been reported. For the study, we tried to develop a technique that was accurate and sensitive enough to detect PF3-like activity of blood. The system we developed was the following: 1) We activated the intrinsic coagulation pathway of commercial standard plasma by ellagic acid. 2) CaCl2, a fixed amount of PF 3 and synthetic thrombin inhibitor MD 805 were added to the reaction mixture. 3) At a fixed time, thrombin activity in the mixture was measured by using S-2238 as a substrate. At full activation of the contact system by ellagic acid, the amount of thrombin formed in a certain time depended on the amount of PF3-like substances such as cephalin, freeze-thawed platelets or ghosts of erythrocytes added to the test system, indicating that PF3-like activity of those substances can be measured by the activity of thrombin generated in a fixed time.(ABSTRACT TRUNCATED AT 250 WORDS)en_US
dc.description.affiliationDepartment of Clinical Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationOpartkiattikul N, Funahara Y, Hijikata-Okunomiya A, Yamaguchi N, Talalak P. Development of a new method for detection of platelet factor 3 like activity. The Southeast Asian Journal of Tropical Medicine and Public Health. 1992 ; 23 Suppl 2(): 47-51en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34591
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshBlood Coagulation Tests --methodsen_US
dc.subject.meshEdetic Acid --diagnostic useen_US
dc.subject.meshErythrocyte Membrane --chemistryen_US
dc.subject.meshErythrocytes, Abnormal --chemistryen_US
dc.subject.meshEvaluation Studies as Topicen_US
dc.subject.meshHemoglobin Een_US
dc.subject.meshHemoglobinopathies --blooden_US
dc.subject.meshHumansen_US
dc.subject.meshPhosphatidylethanolamines --diagnostic useen_US
dc.subject.meshPlatelet Aggregationen_US
dc.subject.meshPlatelet Factor 3 --chemistryen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSplenectomyen_US
dc.subject.meshThrombin --biosynthesisen_US
dc.subject.meshbeta-Thalassemia --blooden_US
dc.titleDevelopment of a new method for detection of platelet factor 3 like activity.en_US
dc.typeJournal Articleen_US
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