Characterization of differentially expressed stress-associated proteins in starch granule development under heat stress in wheat (Triticum aestivum L.).

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dc.contributor.authorKumar, Ranjeet R
dc.contributor.authorSharma, Sushil K
dc.contributor.authorGoswami, Suneha
dc.contributor.authorSingh, G P
dc.contributor.authorSingh, Rajendra
dc.contributor.authorSingh, Khushboo
dc.contributor.authorPathak, Himanshu
dc.contributor.authorRai, Raj D
dc.date.accessioned2013-07-17T05:46:57Z
dc.date.available2013-07-17T05:46:57Z
dc.date.issued2013-04
dc.description.abstractAbiotic stress causes abrupt increase in the expression of stress-associated proteins, which provide tolerance by modulating the defense mechanism of plants. Small heat shock proteins (sHSPs) and anti-oxidant enzymes are important for environmental stress tolerance of the plants. In this study, two full-length cDNAs encoding small heat shock protein (sHSP) and superoxide dismutase (SOD), designated as TasHSP and SODI were identified and characterized from C-306 (thermotolerant) and PBW343 (thermosusceptible) cultivars of wheat (Triticum aestivum L.). An alpha crystalline domain was observed in TasHSP and manganese/iron binding domain in case of SODI. Quantitative real-time PCR showed very high transcript level of TasHSP and SOD in C-306 compared to PBW343 at different stages of growth and against differential heat stress (HS). Under differential HS at milky-dough stage, the fold change in transcript of both TasHSP and SOD was observed maximum in C-306, compared to PBW343. Protein profiling and isoenzymes analysis showed the expression of several heat-stable proteins and prominent isoenzymes of SOD in C-306, compared to PBW343. Scanning electron microscopy (SEM) of starch granules showed globular, well-shaped and more numbers of endospermic cells in C-306, compared to defragmented, irregular shaped and shrunken granules in case of PBW343 under HS treatment (42°C for 2 h). Diurnal change in soluble starch synthase (SSS) activity showed an increase in the activity during afternoon (35°C), compared to morning (29°C) and evening (32°C) in both the cultivars. Under heat stress (42°C for 2 h), a drastic decrease in the SSS activity was observed, due to the thermal denaturation of the enzyme. Thermotolerance capacity analyzed using cell membrane stability (CMS) showed significantly higher CMS in case of C-306, compared to PBW343 at different stages of growth. Findings suggest that abundance of TasHSP and SODI during milky-dough stage plays a very important role in starch granule biosynthesis. The mechanism may be further exploited to develop tolerant wheat cultivar with high quality seeds.en_US
dc.identifier.citationKumar Ranjeet R, Sharma Sushil K, Goswami Suneha, Singh G P, Singh Rajendra, Singh Khushboo, Pathak Himanshu, Rai Raj D. Characterization of differentially expressed stress-associated proteins in starch granule development under heat stress in wheat (Triticum aestivum L.). Indian Journal of Biochemistry & Biophysics. 2013 Apr; 50(2): 126-138.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/147296
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/17100en_US
dc.subjectSmall heat-shock proteinen_US
dc.subjectHeat stressen_US
dc.subjectProtein profilingen_US
dc.subjectIsoenzymesen_US
dc.subjectSuperoxide dismutaseen_US
dc.subjectWheaten_US
dc.subjectSoluble starch synthaseen_US
dc.subjectScanning electron microscopyen_US
dc.subjectTriticum aestivum L.en_US
dc.subject.meshAmino Acid Sequence
dc.subject.meshBase Sequence
dc.subject.meshCell Membrane --metabolism
dc.subject.meshCloning, Molecular
dc.subject.meshDNA, Complementary --metabolism
dc.subject.meshGene Expression Profiling
dc.subject.meshGene Expression Regulation, Plant
dc.subject.meshHeat-Shock Proteins --metabolism
dc.subject.meshHot Temperature
dc.subject.meshIsoenzymes --metabolism
dc.subject.meshMicroscopy, Electron, Scanning
dc.subject.meshModels, Biological
dc.subject.meshMolecular Sequence Data
dc.subject.meshPhylogeny
dc.subject.meshReal-Time Polymerase Chain Reaction --methods
dc.subject.meshSequence Homology, Amino Acid
dc.subject.meshStarch --metabolism
dc.subject.meshStarch Synthase --metabolism
dc.subject.meshSuperoxide Dismutase --metabolism
dc.subject.meshTriticum --metabolism
dc.titleCharacterization of differentially expressed stress-associated proteins in starch granule development under heat stress in wheat (Triticum aestivum L.).en_US
dc.typeArticleen_US
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