Isolation and analysis of circulating tuberculous antigens in Mycobacterium tuberculosis.

dc.contributor.authorShende, Nirajen_US
dc.contributor.authorGupta, Sonikaen_US
dc.contributor.authorUpadhye, Vijayen_US
dc.contributor.authorKumar, Satishen_US
dc.contributor.authorHarinath, Bhaskar Cen_US
dc.date.accessioned2007-07-25en_US
dc.date.accessioned2009-06-01T12:25:05Z
dc.date.available2007-07-25en_US
dc.date.available2009-06-01T12:25:05Z
dc.date.issued2007-07-25en_US
dc.description.abstractBACKGROUND: Serological techniques like enzyme linked immunosorbent assay (ELISA) and immunoblotting are useful for detection of mycobacterial antigens of diagnostic importance in tuberculosis. AIM: To isolate and identify circulating tuberculous antigens reactive with sputum positive and sputum negative pulmonary tuberculosis (PTB) sera. METHODS: Circulating tuberculous antigen was isolated by ammonium sulphate fractionation from the sera of sputum positive and sputum negative (clinically and radiologically diagnosed) PTB cases. The circulating antigen fractions and individual patients' serum samples were resolved by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting was performed using anti M.tb sonicate IgG as a probe to detect antigens. RESULTS: Anti M.tb sonicate IgG was found to be reactive with mycobacterial proteins 170 kDa, 140 kDa, 85 kDa, 55 kDa, 43 kDa, 20 kDa and 16 kDa in the antigen fraction isolated from sputum positive tuberculosis sera by immunoblotting. However only 85 kDa, 55kDa, 43 kDa and 20 kDa antigenic proteins were found to be recognized by anti sonicate IgG in the antigen isolated from sputum negative sera. These observations were further confirmed by analysis of individual S+ and S- PTB serum by immunoblotting. CONCLUSION: Seroreactive studies of circulating tuberculous antigens showed the presence of 170 kDa, 140 kDa, 85 kDa, 55 kDa, 43 kDa, 20 kDa and 16 kDa protein antigens in sputum positive sera, while 85 kDa, 55 kDa, 43 kDa and 20 kDa antigens were found to be present in sputum negative PTB which need further evaluation for their use in serological diagnosis of tuberculosis.en_US
dc.description.affiliationJamnalal Bajaj Tropical Disease Research Centre & Department of Biochemistry, Mahatma Gandhi Institute of Medical Sciences, Sevagram, District: Wardha, (Maharashtra).en_US
dc.identifier.citationShende N, Gupta S, Upadhye V, Kumar S, Harinath BC. Isolation and analysis of circulating tuberculous antigens in Mycobacterium tuberculosis. Indian Journal of Tuberculosis. 2007 Jul; 54(3): 125-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/110535
dc.language.isoengen_US
dc.source.urihttps://medind.nic.in/ibr/ibrai.shtmlen_US
dc.subject.meshAmmonium Sulfate --chemistryen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Bacterial --chemistryen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEnzyme-Linked Immunosorbent Assay --methodsen_US
dc.subject.meshGoatsen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshImmunoglobulin G --chemistryen_US
dc.subject.meshMycobacterium tuberculosis --immunologyen_US
dc.subject.meshPenicillinase --chemistryen_US
dc.subject.meshSerologic Tests --methodsen_US
dc.subject.meshSputum --metabolismen_US
dc.subject.meshTuberculosis, Pulmonary --immunologyen_US
dc.titleIsolation and analysis of circulating tuberculous antigens in Mycobacterium tuberculosis.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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