Cytotoxic Triterpenes and Sterols from Pipturus arborescens (Link) C.B. Rob.

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dc.contributor.authorReyes, Mariquit M De Los
dc.contributor.authorOyong, Glenn G
dc.contributor.authorEbajo, Virgilio D
dc.contributor.authorNg, Vincent Antonio S
dc.contributor.authorShen, Chien-Chang
dc.contributor.authorRagasa, Consolacion Y
dc.date.accessioned2015-12-02T10:34:09Z
dc.date.available2015-12-02T10:34:09Z
dc.date.issued2015-11
dc.description.abstractThe triterpenes, squalene (1), friedelin (2) and a mixture of ursolic acid (3a) and oleanolic acid (3b) in a 2:3 ratio, and a mixture of β-sitosterol (4a) and stigmasterol (4b) in a 2:1 ratio, obtained from the dichloromethane extract of Pipturus arborescens (Link) C.B. Rob., were evaluated for their anti-proliferative activities against three human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast- neonatal (HDFn) using the in vitro PrestoBlue® cell viability assay. The HCT-116 cell line was most susceptible to the compounds and mixtures tested. Triterpene 1 was most cytotoxic against HCT-116 and MCF-7 with IC50 values of 4.21 and 5.92 μg/mL, respectively. Triterpene 2 and the mixture of 3a and 3b were highly anti-proliferative against HCT-116 cells (IC50 of 1.22 and 1.66 μg/mL, respectively) and moderately inhibitory against MCF-7 cells (IC50 of 16.51 and 23.97 μg/mL, respectively). The mixture of 4a and 4b exhibited high cytotoxicity against HCT-116 cells (IC50 of 1.14 μg/mL). Compounds 1-4b showed the least activity against HT-29 cells (IC50 of 11.97 to 52.52 μg/mL). Cytotoxic effect was not observed against HDFn cells (>100 μg/mL). Comparing the effects of 1-4b on the two colon cancer cell lines, the IC50 values of 1-4b against HCT-116 were lower than those of HT-29.en_US
dc.identifier.citationReyes Mariquit M De Los, Oyong Glenn G, Ebajo Virgilio D, Ng Vincent Antonio S, Shen Chien-Chang, Ragasa Consolacion Y. Cytotoxic Triterpenes and Sterols from Pipturus arborescens (Link) C.B. Rob. Journal of Applied Pharmaceutical Science. 2015 Nov; 5(11): 23-30.en_US
dc.identifier.issn2231-3354
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/166873
dc.language.isoenen_US
dc.source.urihttps://www.japsonline.com/abstract.php?article_id=1690en_US
dc.subjectPipturus arborescens (Link) C.B. Rob.en_US
dc.subjectsqualeneen_US
dc.subjectfriedelinen_US
dc.subjectursolic aciden_US
dc.subjectoleanolic aciden_US
dc.subjectβ-sitosterolen_US
dc.subjectstigmasterolen_US
dc.subjectcytotoxicityen_US
dc.subjectMCF-7en_US
dc.subjectHCT-116en_US
dc.subjectHT-29en_US
dc.subjectHDFnen_US
dc.titleCytotoxic Triterpenes and Sterols from Pipturus arborescens (Link) C.B. Rob.en_US
dc.typeArticleen_US
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