Purification of tryptic peptides for mass spectrometry using polyvinylidene fluoride membrane.

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dc.contributor.authorKurien, B Ten_US
dc.contributor.authorMatsumoto, Hen_US
dc.contributor.authorScofield, R Hen_US
dc.date.accessioned2001-08-29en_US
dc.date.accessioned2009-05-27T09:15:21Z
dc.date.available2001-08-29en_US
dc.date.available2009-05-27T09:15:21Z
dc.date.issued2001-08-29en_US
dc.description.abstractA simple procedure for the purification of tryptic peptides, prior to mass spectrometric analysis, using polyvinylidene fluoride membrane (PVDF) is described. The sensitivity of mass spectrometric analysis is such that minor impurities in tryptic peptide digests suppress the signal obtained. However, we obtained useful signal, from a sample that did not yield any spectra earlier, by purifying the sample using PVDF membrane. For this, the tryptic peptide digest was first spotted on the membrane which was then air-dried and washed. Further, the membrane was extracted with trifluoroacetic acid (TFA) and acetonitrile and subjected to mass spectrometric analysis. This procedure enabled us to identify a cross-reactive D1 antigen on the neutrophil surface that bound antibodies that targeted 60 kD Ro autoantigen in systemic lupus erythematosus, an autoimmune disorder.en_US
dc.description.affiliationArthritis and Immunology Program, Oklahoma Medical Research Foundation, Oklahoma City 73104, USA. kurienb@omrf.ouhsc.eduen_US
dc.identifier.citationKurien BT, Matsumoto H, Scofield RH. Purification of tryptic peptides for mass spectrometry using polyvinylidene fluoride membrane. Indian Journal of Biochemistry & Biophysics. 2001 Aug; 38(4): 274-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26946
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshHumansen_US
dc.subject.meshMass Spectrometry --methodsen_US
dc.subject.meshMembranes, Artificialen_US
dc.subject.meshNeutrophils --chemistryen_US
dc.subject.meshPeptides --analysisen_US
dc.subject.meshPolyvinyls --chemistryen_US
dc.subject.meshTrypsin --metabolismen_US
dc.titlePurification of tryptic peptides for mass spectrometry using polyvinylidene fluoride membrane.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeResearch Support, U.S. Gov't, P.H.S.en_US
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