Anticholinesterase, antioxidant, and neuroprotective effects of Tripleurospermum disciforme and Dracocephalum multicaule.
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Date
2014-07
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Abstract
Background: Nowadays, owing to medicinal plants as a candidate to obtain promising new medicinal agents, there is a renewed
interest in the use of these natural sources for drug development. Objective: In the present study, we aimed to assess the
anticholinesterase, antioxidant, and neuropotective effects of Tripleurospermum disciforme and Dracocephalum multicaule
extracts. Materials and Methods: Methanolic extract of the plants was prepared by maceration method. Anticholinesterase
effect of different concentrations of the plants was studied by colorimetric method and antioxidant activity was evaluated
using diphenypicrylhydrazil (DPPH) assay. Protective effect of the extracts against amyloid b (Ab)‑induced toxicity in
PC12 cells was determined by MTT (3‑(4,5‑dimethyl thiazol‑2‑yl)‑2,5‑diphenyl tetrazolium bromide) method. Results: Both
T. disciforme and D. multicaule extracts could inhibit acetylcholinesterase (AChE) in a dose‑dependent manner. The highest
inhibition occurred at 5 μg/ml (71.18 ± 4.9 and 79.06 ± 3.1% inhibition respectively by T. disciforme and D. multicaule)
in comparison to tacrine (86.37 ± 3.24%). The greatest DPPH inhibition of T. disciforme and D. multicaule was shown at
800 μg/ml (89.04 ± 3.9 and 78.5 ± 3.7%, respectively). None of tested extracts induced protection against bA toxicity in PC12
cell. Conclusion: Although the results indicated anticholinesterase and antioxidant of the T. disciforme and D. multicaule,
further specific studies and scientific validity are needed.
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Keywords
Anticholinesterase, antioxidant, Dracocephalum multicaule, Tripleurospermum disciforme
Citation
Mandegary Ali, Soodi Maliheh, Sharififar Fariba, Ahmadi Samira. Anticholinesterase, antioxidant, and neuroprotective effects of Tripleurospermum disciforme and Dracocephalum multicaule. Journal of Ayurveda and Integrative Medicine. 2014 July-Sept; 5(3): 162-166.