Reliability of Kirby-Bauer disk diffusion method for detecting meropenem resistance among non-fermenting gram-negative bacilli.

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dc.contributor.authorJoseph, Noyal M
dc.contributor.authorSistla, Sujatha
dc.contributor.authorDutta, Tarun K
dc.contributor.authorBadhe, Ashok S
dc.contributor.authorRasitha, Desdemona
dc.contributor.authorParija, Subhash C
dc.date.accessioned2012-10-11T08:41:24Z
dc.date.available2012-10-11T08:41:24Z
dc.date.issued2011-07
dc.description.abstractBackground: Meropenem is empirically used as a last resort for the treatment of infections by non-fermenting gram-negative bacilli (NFGNB). Minimum inhibitory concentration (MIC) determined using agar or broth dilution methods is widely used for testing meropenem resistance. However, it is not possible in resource-poor settings. Aim: A prospective study was performed to evaluate the reliability of Kirby-Bauer disk diffusion (KBDD) method for detecting meropenem resistance among NFGNB. Materials and Methods: A total of 146 NFGNB consisting of 56 Acinetobacter baumannii, 24 Acinetobacter lwoffii, 48 Pseudomonas aeruginosa and 18 Pseudomonas spp. were included in the study. All the isolates were tested simultaneously by both KBDD method and agar dilution method. Results: Very major errors were not observed with A. baumannii, A. lwoffii and P. aeruginosa, while other Pseudomonas spp. showed a very major error rate of about 5.6%. The major error rates observed with A. baumannii, A. lwoffii, P. aeruginosa and Pseudomonas spp. were 1.8%, 0%, 2.1% and 28.6%, respectively. All the isolates showed a good correlation between zone diameters (KBDD method) and MICs (agar dilution method). The sensitivity and specificity of KBDD method for detecting meropenem resistance was above 90% for all the NFGNB except Pseudomonas spp. Conclusions: The KBDD method can be reliably used for routine testing of meropenem resistance in A. baumannii, A. lwoffii and P. aeruginosa. However, further studies are needed before employing this technique for detecting meropenem resistance in Pseudomonas spp.en_US
dc.identifier.citationJoseph Noyal M, Sistla Sujatha, Dutta Tarun K, Badhe Ashok S, Rasitha Desdemona, Parija Subhash C. Reliability of Kirby-Bauer disk diffusion method for detecting meropenem resistance among non-fermenting gram-negative bacilli. Indian Journal of Pathology & Microbiology. 2011 Jul-Sept 54(3): 556-560.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/142042
dc.language.isoenen_US
dc.source.urihttps://www.ijpmonline.org/article.asp?issn=0377-4929;year=2011;volume=54;issue=3;spage=556;epage=560;aulast=Josephen_US
dc.subjectAcinetobacteren_US
dc.subjectagar dilutionen_US
dc.subjectdisk diffusionen_US
dc.subjectminimum inhibitory concentrationen_US
dc.subjectPseudomonasen_US
dc.subjectzone diameteren_US
dc.subject.meshAcinetobacter --drug effects
dc.subject.meshAnti-Bacterial Agents --pharmacology
dc.subject.meshDiagnostic Errors --statistics & numerical data
dc.subject.meshDisk Diffusion Antimicrobial Tests --methods
dc.subject.meshHumans
dc.subject.meshProspective Studies
dc.subject.meshPseudomonas --drug effects
dc.subject.meshThienamycins --pharmacology
dc.subject.meshbeta-Lactam Resistance
dc.titleReliability of Kirby-Bauer disk diffusion method for detecting meropenem resistance among non-fermenting gram-negative bacilli.en_US
dc.typeArticleen_US
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