DNA extraction and amplification of 10-day, room-temperature blood samples.

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dc.contributor.authorSasanakul, Wen_US
dc.contributor.authorChuansumrit, Aen_US
dc.contributor.authorRurgkhum, Sen_US
dc.contributor.authorUdomsubpayakul, Uen_US
dc.contributor.authorHathirat, Pen_US
dc.date.accessioned2009-05-27T21:43:28Z
dc.date.available2009-05-27T21:43:28Z
dc.date.issued1999-11-24en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractDNA was serially studied in 20 samples of buffy coat stored at room temperature. Each sample was divided into 5 equal volumes, namely D0, D3, D5, D7 and D10. DNA extraction was performed on days 0, 3, 5, 7 and 10 after blood collection. The mean ratio of OD260/OD280 of the DNA obtained from D0 to D10 ranged from 1.77 to 1.79, and the mean amounts of the DNA obtained from D0 to D10 ranged from 602 to 740 ng/ul. There were no significant differences in the mean ratio and amounts of DNA obtained among these samples (p > 0.05). Subsequently, amplification was successfully performed from this template DNA to yield products of 1.4 kb and 142 bp at the sites associated with beta globin and factor VIII genes, respectively. These findings suggest the possibility of sending blood samples for DNA analysis by mail, or no ice is required during transportation.en_US
dc.description.affiliationResearch Center, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationSasanakul W, Chuansumrit A, Rurgkhum S, Udomsubpayakul U, Hathirat P. DNA extraction and amplification of 10-day, room-temperature blood samples. Journal of the Medical Association of Thailand. 1999 Nov; 82 Suppl 1(): S186-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/45291
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshAdulten_US
dc.subject.meshBlood Chemical Analysisen_US
dc.subject.meshDNA --blooden_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Amplificationen_US
dc.subject.meshGenetic Techniquesen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshSpecimen Handlingen_US
dc.subject.meshTemperatureen_US
dc.titleDNA extraction and amplification of 10-day, room-temperature blood samples.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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