Optimization of cellulolytic enzyme production by thermophilic fungus Thermoascus aurantiacus using response surface methodology

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dc.contributor.authorSingh, Anitaen_US
dc.contributor.authorBajar, Somviren_US
dc.date.accessioned2020-01-02T06:23:23Z
dc.date.available2020-01-02T06:23:23Z
dc.date.issued2019-10
dc.description.abstractIn the present study, thermophilic fungus Thermoascus aurantiacus, a local isolate, was used for production of cellulolytic enzymes using rice straw (RS) as substrate under submerged fermentation using Box-Behnken Design (BBD) of Response Surface Methodology (RSM). Themophilic fungus T. aurantiacus grew very well at 50-55°C temperature. BBD was used to study the influence of process parameters, pH (4-6), temperature (40-60°C) and substrate concentration (2-8 g/L) and their interactive effect on cellulase enzyme production. The R2 value 0.94 for Filter paper activity and 0.95 for carboxymethyl cellulase activity indicate that the model is appropriate and replicated to predict the effect of pH, temperature and substrate concentration on both enzyme activities. Under optimized conditions, 6.1 (U/gds) FPase activity and 28.2 (U/gds) CMCase activity was observed.en_US
dc.identifier.affiliationsDepartment of Environmental Sciences, Central University of Jammu, Jammu- 181 143, Jammu and Kashmir, Indiaen_US
dc.identifier.affiliationsDepartment of Environmental Sciences, Central University of Haryana, Mahendergarh- 123 031, Haryana, Indiaen_US
dc.identifier.citationSingh Anita, Bajar Somvir. Optimization of cellulolytic enzyme production by thermophilic fungus Thermoascus aurantiacus using response surface methodology. Indian Journal of Biochemistry and Biophysics. 2019 Oct; 56(5): 399-403en_US
dc.identifier.issn0975-0959
dc.identifier.issn0301-1208
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/190070
dc.languageenen_US
dc.publisherNISCAIR-CSIR, Indiaen_US
dc.relation.issuenumber5en_US
dc.relation.volume56en_US
dc.source.urihttps://nopr.niscair.res.in/handle/123456789/50505en_US
dc.titleOptimization of cellulolytic enzyme production by thermophilic fungus Thermoascus aurantiacus using response surface methodologyen_US
dc.typeJournal Articleen_US
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