Epidemiological study of sulfonamide and trimethoprim resistance genes in Enterobacteriaceae.

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dc.contributor.authorPanbangred, Wen_US
dc.contributor.authorJayanetra, Pen_US
dc.contributor.authorPilantanapak, Aen_US
dc.date.accessioned2009-05-27T15:59:38Z
dc.date.available2009-05-27T15:59:38Z
dc.date.issued1990-06-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractSulfonamide (Su) and trimethoprim (Tp) resistance are known to caused by the production of drug resistant dihydropteroate synthase (DHPS) and dihydrofolate reductase (DHFR), respectively. Sulfonamide and trimethoprim are often used in combination under the name cotrimoxazole. Cotrimoxazole resistance in various enteric bacteria isolated at Ramathibodi Hospital was studied. The rate of resistance from 1984-1989 of many genera was rather constant at 40%-60% except in Shigella spp in which the rate increased rapidly in 1987 till 1989. Seventy-five percent of Su-Tp resistant (Sur-Tpr) bacteria were also found to be resistant to other drugs such as ampicillin, aminoglycosides, tetracycline and chloramphenicol in addition to cotrimoxazole. Two hundred and forty Su-Tp resistant strains were analysed for the presence of type I and II dihydropteroate synthase as well as type I and V dihydrofolate reductase genes by hybridization with the corresponding gene probes. Type I DHPS gene predominated in Su-Tp resistant bacteria at 60.8% whereas type II DHPS was found in only 25%. Some strains (11.7%) had both genotypes but 2.5% did not have any. In the trimethoprim resistance study, the DHFR type I gene was also found more frequently (30%) whereas type V DHFR was only 19%. The remaining of Tp resistance (51%) was unclassified. The coexistence of Su and Tp resistance genes of each type was investigated among 118 Su and Tp resistant strains. It was found that type I DHPS gene was found together with either type I or V DHFR gene and type II DHPS was found with type I DHFR gene at about the same rate (28.9%, 27.1% and 26.3%, respectively). However, the presence of type II DHPS together with type V DHFR was rather low, only 5.9% of isolates were found to have both types of genes.en_US
dc.description.affiliationDepartment of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.en_US
dc.identifier.citationPanbangred W, Jayanetra P, Pilantanapak A. Epidemiological study of sulfonamide and trimethoprim resistance genes in Enterobacteriaceae. The Southeast Asian Journal of Tropical Medicine and Public Health. 1990 Jun; 21(2): 175-84en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/34160
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshDNA Probesen_US
dc.subject.meshDihydropteroate Synthase --biosynthesisen_US
dc.subject.meshDrug Resistance, Microbial --geneticsen_US
dc.subject.meshEnterobacteriaceae --drug effectsen_US
dc.subject.meshEnterobacteriaceae Infections --drug therapyen_US
dc.subject.meshHumansen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshPlasmidsen_US
dc.subject.meshTetrahydrofolate Dehydrogenase --biosynthesisen_US
dc.subject.meshThailand --epidemiologyen_US
dc.subject.meshTrimethoprim Resistance --geneticsen_US
dc.subject.meshTrimethoprim-Sulfamethoxazole Combination --pharmacologyen_US
dc.titleEpidemiological study of sulfonamide and trimethoprim resistance genes in Enterobacteriaceae.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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