Capsaicin fails to produce changes in contractile tension in large gut of neonate rats

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dc.contributor.authorSingh, Sen_US
dc.contributor.authorSharma, Pen_US
dc.contributor.authorDixit, Den_US
dc.contributor.authorMandal, MB.en_US
dc.date.accessioned2023-08-19T04:57:22Z
dc.date.available2023-08-19T04:57:22Z
dc.date.issued2023-03
dc.description.abstractObjectives: Capsaicin, the most pungent constituent of chilli pepper (Capsicum annuum L.), is known to alter the physiological activity of the gut. Capsaicin mediates its action through a transient receptor potential vanilloid type 1 (TRPV1) channel. The action of capsaicin on gut smooth muscle varies from segment to segment in different species. The earlier studies were carried out in adult animals only, and its status in the neonate gut, which is in a development stage, is not known. Objective: Therefore, the present study was done to assess the effect of capsaicin on the large gut of neonates. Materials and Methods: In an organ bath preparation, isometric contractions were recorded from segments of dissected rat colon and rectum. The gut segments were exposed to cumulative concentrations of capsaicin (0.01 nM–3 µM) and a capsaicin-induced contractile response was observed. TRPV1 receptor antagonist capsazepine (1 µM) and a nitric oxide synthase inhibitor, L-NAME (100 µM), were used to assess their blocking effect on capsaicin-induced contractile response. Results: Capsaicin raised contractile tension in the colon and rectum of adult rats but not in neonate rats. In adult rats, capsazepine pre-treatment (1 µM) failed to block the capsaicin-induced response in the colon, but in the lower concentrations, it increased contractile tension in the rectum. Pre-application of L-NAME (100 µM) potentiated capsaicin-induced response in the adult rectum and neonate’s colon but had no effect in the neonate rectum and adult colon. Capsaicin with a low concentration (0.01 nM–0.01 µM) increased contractile frequency in both the colon and rectum of adult rats. However, the effect of capsaicin on frequency was abolished at higher concentrations (0.01 µM–3 µM). A capsaicin-evoked change in contractile frequency in adult rats was blocked by capsazepine and L-NAME. At lower concentrations (0.01 nM–0.01 µM), capsaicin did not show any change in frequency in the neonatal colon, while a decrease in contractile frequency was observed with the higher concentrations (0.1 µM–3 µM) of capsaicin. In neonates, capsazepine pre-treatment produced changes in frequency for both the colon and rectum. However, pre-application of L-NAME decreased frequency in the neonate rectum but not in the colon. Conclusion: Capsaicin-induced changes in contractile activity may or may not involve TRPV1 or the Nitric Oxide (NO) pathway, depending on the part of the large gut and developmental maturity.en_US
dc.identifier.affiliationsDepartment of Physiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, Indiaen_US
dc.identifier.citationSingh S, Sharma P, Dixit D, Mandal MB.. Capsaicin fails to produce changes in contractile tension in large gut of neonate rats. Indian Journal of Physiology and Pharmacology. 2023 Mar; 67(1): 36-43en_US
dc.identifier.issn0019-5499
dc.identifier.issn2582-2799
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223976
dc.languageenen_US
dc.publisherScientific Scholar on behalf of Association of Physiologists and Pharmacologists of Indiaen_US
dc.relation.issuenumber1en_US
dc.relation.volume67en_US
dc.source.urihttps://doi.org/10.25259/IJPP_437_2022en_US
dc.subjectCapsaicinen_US
dc.subjectColonen_US
dc.subjectRectumen_US
dc.subjectGut contractilityen_US
dc.subjectCapsazepineen_US
dc.titleCapsaicin fails to produce changes in contractile tension in large gut of neonate ratsen_US
dc.typeJournal Articleen_US
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