Serum eosinophil cationic protein determination in asthmatic children-effect of different collecting tubes used for blood sampling.

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dc.contributor.authorLin, S Jen_US
dc.contributor.authorChao, H Cen_US
dc.contributor.authorYan, D Cen_US
dc.contributor.authorHuang, J Len_US
dc.date.accessioned2009-05-27T17:12:18Z
dc.date.available2009-05-27T17:12:18Z
dc.date.issued1999-12-04en_US
dc.descriptionPublished by the Allergy and Immunology Society of Thailand.en_US
dc.description.abstractWe compared the effect of using 2 different serum collecting tubes, serum separation tubes (SST, with clot activator and gel barrier) and conventional glass tubes (with no additives), on circulating levels of eosinophil cationic protein (ECP) in asthmatic children and controls. The serum ECP values obtained from both SST and glass tubes were significantly higher in asthmatic children than in corresponding controls. ECP values were higher in serum samples using SST than in those using glass tubes (P<0.01), while no difference was found between the two in controls. ECP levels correlated with peripheral eosinophil counts, for SST samples and glass tube samples alike. The difference in ECP levels between these two tubes also correlated with circulating eosinophil counts (r = 0.62, P = 0.004) After 18-hour storage at room temperature, the ECP values increased significantly in samples obtained from asthmatic children. No difference in ECP values between SST samples and glass tube samples was found for 18 hour samples. Thus, ECP levels obtained from SST samples and glass tube samples, though reliable, should not be directly compared, especially in asthmatic children with eosinophilia.en_US
dc.description.affiliationDepartment of Pediatrics, Chang Gung Children's Hospital, Taiwan.en_US
dc.identifier.citationLin SJ, Chao HC, Yan DC, Huang JL. Serum eosinophil cationic protein determination in asthmatic children-effect of different collecting tubes used for blood sampling. Asian Pacific Journal of Allergy and Immunology. 1999 Dec; 17(4): 269-73en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36680
dc.language.isoengen_US
dc.subject.meshAsthma --blooden_US
dc.subject.meshBlood Proteins --analysisen_US
dc.subject.meshBlood Specimen Collection --methodsen_US
dc.subject.meshChilden_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshEosinophil Granule Proteinsen_US
dc.subject.meshEosinophilsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshLeukocyte Counten_US
dc.subject.meshMaleen_US
dc.subject.meshRibonucleasesen_US
dc.subject.meshTime Factorsen_US
dc.titleSerum eosinophil cationic protein determination in asthmatic children-effect of different collecting tubes used for blood sampling.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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