2-Methoxy-1,4-Naphthoquinone (MNQ) Suppresses Protein Kinase C βI, δ, and ζ Expression in Raji Cells.
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Date
2015-08
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Abstract
Protein Kinase C (PKC) is widely documented to be involved in the regulation of cancer cell growth,
proliferation, survival, inflammation and apoptosis. This study evaluates the capability of 2-Methoxy-1,4-
Naphthoquinone (MNQ) in regulating PMA-induced PKC expression in human Burkitt’s lymphoma cell line
(Raji cells). MNQ exhibited stronger anti-tumour-promoting activity than genistein based on the inhibitory assay
of Epstein-Barr virus (EBV) activation. The IC50 values attained were 2.92 and 7.40 μM, respectively. The
suppressive effects of MNQ on PKC expression was determined by using the PepTag® non-radioactive detection
of PKC assay. The IC50 values achieved for staurosporine and rottlerin (standard control), and MNQ were 0.01,
6.38, and 13.13 μM, respectively. These preliminary results indicate that MNQ specifically suppressed the
expression of PKC βI, δ, and ζ in a concentration-dependent manner in Raji cells.
Description
Keywords
Protein kinase C, Anti-tumour-promoting, 2-Methoxy-1, 4-Naphthoquinone, Cancer chemoprevention
Citation
Yew Wong Teck, Kitson Liew, Hock Anthony Ho Siong, Chiew Gan Seng, Mooi Lim Yang. 2-Methoxy-1,4-Naphthoquinone (MNQ) Suppresses Protein Kinase C βI, δ, and ζ Expression in Raji Cells. Journal of Applied Pharmaceutical Science. 2015 Aug; 5(8): 1-5.