The effects of microRNA-1224-5p on hepatocellular carcinoma tumor endothelial cells

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dc.contributor.authorHu, Chaoen_US
dc.contributor.authorCheng, Xien_US
dc.contributor.authorMingYu, Qien_US
dc.contributor.authorWang, Xin Baoen_US
dc.contributor.authorShen, Shi Qiangen_US
dc.date.accessioned2020-11-18T10:07:37Z
dc.date.available2020-11-18T10:07:37Z
dc.date.issued2019-04
dc.description.abstractAim: The aim of this study was to investigate the effect of microRNA-1224-5p (miR-1224-5p) on tumor endothelial cells (TECs) of human hepatocellular carcinoma (HCC). Subjects and Methods: Oligonucleotides were chemically synthesized and transfected into TECs using Lipofectamine 2000. TECs were divided into three groups, namely a control (CON) group without transfection, a negative control (NC) group transfected with negative control oligonucleotides and green fluorescent protein (GFP), and a micro-up (MU) group transfected with miR-1224-5p mimic and GFP. The expression of miR-1224-5p was quantified via quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The proliferation of TECs was detected using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and the optical density value at 490 nm was measured after every 24 h. Apoptosis was detected via flow cytometry using a 7-aminoactinomycin/APC Annexin V kit. The migration and invasion of TECs were detected using transwell assay. The tube formation ability was evaluated using the tube formation assay. Results: Oligonucleotides were successfully transduced into TECs, and the expression of miR-1224-5p was specifically upregulated. The results of qRT-PCR analysis showed that the expression of miR-1224-5p was significantly upregulated in the MU group (2–ΔΔCt = 3.27 ± 0.15) than in the CON group (2–ΔΔCt = 1) and NC group (2–ΔΔCt = 1.08 ± 0.11) (P < 0.01). The results of MTT assay showed that the cell proliferation was significantly inhibited in the MU group at four time points than in the CON and NC groups (P < 0.01). Flow cytometry analysis revealed the significant increase in apoptosis of cells from the MU group (19.29% ± 0.95%) than those from the CON (8.73% ± 0.64%) and NC (9.51% ± 0.56%) (P < 0.01) groups. The migration ability was significantly inhibited in MU group (51.0 ± 3.6) as compared with CON (77.7 ± 2.5) and NC (79.2 ± 3.5) groups (P < 0.01). The invasion ability of TECs was significantly inhibited in MU group (9.8 ± 1.3) than in CON (15.8 ± 0.8) and NC (15.4 ± 0.9) groups (P < 0.01). The ability of tube formation of TECs was completely inhibited in MU group but remained unaffected in CON and NC groups. Conclusions: miR-1224-5p may serve as a potential tumor suppressor in HCC. Upregulation in miR-1224-5p expression may decrease proliferation, induce apoptosis, inhibit migration and invasion, and suppress tube formation in TECs of human HCCen_US
dc.identifier.affiliationsDepartment of Abdominal Surgical Oncology, Zhejiang Cancer Hospital, Hangzhou, Chinaen_US
dc.identifier.affiliationsDepartment of Abdominal Surgical Oncology, Zhejiang Cancer Hospital, Hangzhou, Chinaen_US
dc.identifier.affiliationsDepartment of Abdominal Surgical Oncology, Zhejiang Cancer Hospital, Hangzhou, Chinaen_US
dc.identifier.affiliationsDepartment of Abdominal Surgical Oncology, Zhejiang Cancer Hospital, Hangzhou, Chinaen_US
dc.identifier.affiliationsDepartment of General Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, Chinaen_US
dc.identifier.citationHu Chao, Cheng Xi, MingYu Qi, Wang Xin Bao, Shen Shi Qiang. The effects of microRNA-1224-5p on hepatocellular carcinoma tumor endothelial cells. Journal of Cancer Research and Therapeutics. 2019 Apr; 15(2): 329-335en_US
dc.identifier.issn0973-1482
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/213618
dc.languageenen_US
dc.publisherWolters Kluwer India Pvt. Ltd.en_US
dc.relation.issuenumber2en_US
dc.relation.volume15en_US
dc.source.urihttps://dx.doi.org//10.4103/jcrt.JCRT_40_18en_US
dc.subjectHepatocellular carcinomaen_US
dc.subjectmicroRNA-1224-5pen_US
dc.subjecttumor endothelial cellen_US
dc.titleThe effects of microRNA-1224-5p on hepatocellular carcinoma tumor endothelial cellsen_US
dc.typeJournal Articleen_US
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