Comparison of various conventional methods with a polymerase chain reaction assay for detecting methicillin-resistant & susceptible Staphylococcus aureus strains.

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dc.contributor.authorPrasad, K Nen_US
dc.contributor.authorKumar, Ren_US
dc.contributor.authorTiwari, D Pen_US
dc.contributor.authorMishra, K Ken_US
dc.contributor.authorAyyagari, Aen_US
dc.date.accessioned2000-12-15en_US
dc.date.accessioned2009-05-27T08:00:08Z
dc.date.available2000-12-15en_US
dc.date.available2009-05-27T08:00:08Z
dc.date.issued2000-12-15en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Methicillin-resistant Staphylococcus aureus (MRSA), a major nosocomial pathogen world-wide, is often difficult to detect due to the heterogeneous nature of expression of oxacillin resistance. In the present study, various conventional methods were compared with polymerase chain reaction on 106 clinical isolates of Staph. aureus for detection of oxacillin resistance. METHODS: A total of 106 clinical isolates of Staph. aureus were tested for oxacillin resistance by disc diffusion, screen agar plates (3 micrograms and 6 micrograms/ml of oxacillin), oxacillin broth (3 micrograms/ml) and mecA based PCR. RESULTS: PCR detected mecA gene amplified product of 604 bp in 57 strains. Disc diffusion failed to detect 7 mecA positive strains but identified 5 mecA negative strains as oxacillin resistant. Screen agar 3 micrograms, screen agar 6 micrograms and oxacillin broth 3 micrograms detected 55, 53 and 55 respectively of the 57 mecA positive strains; however, they also falsely identified 5, 3 and 3 strains of mecA negative strains respectively as oxacillin resistant. The sensitivity, specificity and accuracy of disc diffusion, 3 micrograms screen agar, 6 micrograms screen agar and 3 micrograms oxacillin broth against PCR as gold standard were as follows: 87.7, 89.9 and 88.7 per cent; 96.5, 89.8 and 93.4 per cent; 93.0, 93.9 and 93.4 per cent; 96.5, 93.9 and 95.3 per cent respectively. INTERPRETATION & CONCLUSIONS: The present study demonstrated that disc diffusion test was least reliable and 3 micrograms broth had the highest sensitivity and specificity when compared with PCR for detection of oxacillin resistance. Because of variations among the methods, a combination of tests should be used for the accurate detection of MRSA till new guidelines by an appropriate body are formulated.en_US
dc.description.affiliationDepartment of Microbiology, Sanjay Gandhi Post-Graduate Institute of Medical Sciences, Lucknow. knprasad@sgpgi.ac.inen_US
dc.identifier.citationPrasad KN, Kumar R, Tiwari DP, Mishra KK, Ayyagari A. Comparison of various conventional methods with a polymerase chain reaction assay for detecting methicillin-resistant & susceptible Staphylococcus aureus strains. Indian Journal of Medical Research. 2000 Dec; 112(): 198-202en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/22877
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshDNA Primersen_US
dc.subject.meshMethicillin Resistanceen_US
dc.subject.meshPolymerase Chain Reaction --methodsen_US
dc.subject.meshStaphylococcus aureus --drug effectsen_US
dc.titleComparison of various conventional methods with a polymerase chain reaction assay for detecting methicillin-resistant & susceptible Staphylococcus aureus strains.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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