Determination of Benzalkonium Chloride in Ophthalmic Solutions by Stability-Indicating HPLC Method: Application to a Stability Study.

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dc.contributor.authorAlAani, Hashem
dc.contributor.authorAlNukkary, Yasmin
dc.date.accessioned2016-09-07T05:34:19Z
dc.date.available2016-09-07T05:34:19Z
dc.date.issued2016-05
dc.description.abstractA simple HPLC method for determination of Benzalkonium chloride in various ophthalmic solutions was developed. The chromatographic analysis was achieved using CN column (250 mm, 4.6 mm i.d., 5 μm) with isocratic mobile phase consisting of acetonitrile - phosphate buffer (pH 5.5; 0.05 M) (70:30, v/v) at a flow rate of 1 mL/minute. The column temperature was maintained at 25°C and the detection wavelength was 210 nm. The proposed HPLC method was successfully validated according to the ICH guideline and proved to be stability-indicating. This method was applied to quantify Benzalkonium chloride during in-use stability study of two ophthalmic solutions. Antimicrobial effectiveness of Benzalkonium chloride in these solutions was also evaluated. The developed method is suitable for the routine analysis of Benzalkonium chloride in many ophthalmic solutions as well as for the stability studies.en_US
dc.identifier.citationAlAani Hashem, AlNukkary Yasmin. Determination of Benzalkonium Chloride in Ophthalmic Solutions by Stability-Indicating HPLC Method: Application to a Stability Study. Journal of Applied Pharmaceutical Science. 2016 May; 6(5): 80-89.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/177001
dc.language.isoenen_US
dc.source.urihttps://www.japsonline.com/abstract.php?article_id=1864en_US
dc.subjectBenzalkonium chlorideen_US
dc.subjectOphthalmic solutionsen_US
dc.subjectHPLCen_US
dc.subjectStability-indicatingen_US
dc.subjectIn-use stabilityen_US
dc.titleDetermination of Benzalkonium Chloride in Ophthalmic Solutions by Stability-Indicating HPLC Method: Application to a Stability Study.en_US
dc.typeArticleen_US
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