Optimization of lovastatin production by Fusarium nectrioides (MH173849) using response surface methodology and fuzzy logic system

Simple item page
dc.contributor.authorSenthamarai, M.en_US
dc.contributor.authorKannan, K.P.en_US
dc.date.accessioned2020-11-18T10:16:19Z
dc.date.available2020-11-18T10:16:19Z
dc.date.issued2019-09
dc.description.abstractAim: To enhance the productivity of lovastatin from Fusarium nectrioides isolate with liquid cheese whey as a major carbon source and to optimize the media components using Response Surface Methodology (RSM) and Fuzzy Logic System (FLS). Methodology: Euphorbia hirta was collected, surface sterilized and incubated on potato dextrose agar medium amended with ampicillin and streptomycin sulphate. F. nectrioides was isolated from E. hirta and identified using morphological and molecular methods. Primarily, media components were screened by Plackett Burman design (PBD). Further, the effect of significant nutrients was predicted using RSM and FLS and compared with experimental yield. Results: Molecular identification by gene sequencing confirmed the isolate to be F. nectrioides, given an accession number (MH173849) the sequence was submitted in the gene bank. PBD revealed that peptonized milk (which is an enzymic digest of high grade skimmed milk powder), corn steep liquor, liquid cheese whey and histidine were significant variables. The optimum levels of these significant variables in different combinations were studied by RSM in which the predicted yield of lovastatin was 1.2 gl-1. Further, it was analyzed by FLS with 14 set of fuzzy rules and the maximum production obtained was 1.8 g100 ml-1 which was closer to the experimental yield of 1.75 g100 ml-1. Therefore, compared to RSM, FLS was more suitable technique to determine the optimum levels of significant nutrients for enhanced lovastatin production. Interpretation: This study suggests that F. nectrioides (MH173849) can be used as a potent producer of lovastatin and the production highly influenced by glucose, corn steep liquor, liquid cheese whey and histidine.en_US
dc.identifier.affiliationsDepartment of Biotechnology,Bannari Amman Institute ofTechnology, Sathyamangalam,Erode-638 401, Indiaen_US
dc.identifier.citationSenthamarai M., Kannan K.P.. Optimization of lovastatin production by Fusarium nectrioides (MH173849) using response surface methodology and fuzzy logic system. Journal of Environmental Biology . 2019 Sep; 40(5): 1036-1044en_US
dc.identifier.issn0254-8704
dc.identifier.issn2394-0379
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/214623
dc.languageenen_US
dc.publisherTriveni Enterprisesen_US
dc.relation.issuenumber5en_US
dc.relation.volume40en_US
dc.source.urihttps://www.jeb.co.in/journal_issues/201909_sep19/paper_08.pdfen_US
dc.subjectCheese Liquid wheyen_US
dc.subjectEuphorbia hirtaen_US
dc.subjectFusarium nectrioidesen_US
dc.subjectFuzzy Logic systemen_US
dc.subjectLovastatinen_US
dc.titleOptimization of lovastatin production by Fusarium nectrioides (MH173849) using response surface methodology and fuzzy logic systemen_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
jeb2019v40n5p1036.pdf
Size:
3.82 MB
Format:
Adobe Portable Document Format
Download
Collections
Journal of Environmental Biology