Anti-ulcer & antioxidant activities of Hedranthera barteri {(Hook F.) Pichon} with possible involvement of H+, K+ ATPase inhibitory activity.

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dc.contributor.authorOnasanwo, Samuel A
dc.contributor.authorSingh, Neetu
dc.contributor.authorOlaleye, Samuel B
dc.contributor.authorMishra, Vaibhav
dc.contributor.authorPalit, Gautam
dc.date.accessioned2011-12-01T10:26:50Z
dc.date.available2011-12-01T10:26:50Z
dc.date.issued2010-10
dc.description.abstractBackground & objective: Hedranthera barteri (HB) is used in folk medicine as a vermifuge, laxative and an anti-inflammatory agent. The aim of this study was to evaluate the anti-ulcer and antioxidant properties of the dichloromethane fraction of HB root (DMHBR). Methods: Anti-ulcerogenic activity was assessed in cold-restraint (CRU), aspirin (ASP), alcohol (AL), pyloric ligation (PL) induced gastric ulcer models in rats and histamine-induced duodenal ulcer (HST) in guinea pigs. The effect of DMHBR (100 mg/kg) on gastric juice for free and total acidity, peptic activity and mucin secretion, using the pylorus ligated model, were evaluated. The H+, K+-ATPase activity was assayed in gastric microsomes, spectrophotometrically. The in vitro anti-oxidant assays were explored through DPPH, nitric oxide, hydroxyl radical, superoxide anion scavenging assays. Results: DMHBR reduced the incidence of ulcers in CRU (63.3%), PL (58.5%), ASP (52.7%), HST (75.0%) and AL (53.87%). Also, reductions were observed in the free acidity (49.4%), total acidity (45.8%) and peptic activity (32.9%) with increase in the mucin secretion by 81.6 per cent. DMHBR (60-100 μg/ml) inhibited the H+,K+-ATPase activity with IC50 of 89.64 μg/ml compared with omeprazole (10-50 μg/ml ) with IC50 of 32.26 μg/ml. DMHBR showed antioxidant activity with IC50 values of DPPH (397.69 μg/ml), nitric oxide (475.88 μg/ml), hydroxyl radical (244.22 μg/ml) and superoxide anion radical (285.20 μg/ml). Interpretation & conclusion: DMHBR showed anti-ulcer activity against experimentally-induced peptic ulcer models and exhibited both cytoprotective and anti-secretory property. It exhibited a proton pump inhibition activity and its anti-ulcer properties may be partly ascribed to its antioxidant activities.en_US
dc.identifier.citationOnasanwo Samuel A, Singh Neetu, Olaleye Samuel B, Mishra Vaibhav, Palit Gautam. Anti-ulcer & antioxidant activities of Hedranthera barteri {(Hook F.) Pichon} with possible involvement of H+, K+ ATPase inhibitory activity. Indian Journal of Medical Research. 2010 Oct; 132(4): 442-449.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135607
dc.language.isoenen_US
dc.source.urihttps://icmr.nic.in/ijmr/2010/October/15.pdfen_US
dc.subjectAntioxidanten_US
dc.subjectH+, K+-ATPaseen_US
dc.subjectHedranthera barterien_US
dc.subjectomeprazoleen_US
dc.subjectpeptic ulceren_US
dc.subject.meshAnimals
dc.subject.meshAnti-Ulcer Agents --pharmacology
dc.subject.meshAntioxidants --pharmacology
dc.subject.meshApocynaceae --chemistry
dc.subject.meshGastric Juice --drug effects
dc.subject.meshH(+)-K(+)-Exchanging ATPase --antagonists & inhibitors
dc.subject.meshMethylene Chloride
dc.subject.meshMicrosomes --metabolism
dc.subject.meshPhytotherapy --methods
dc.subject.meshPlant Extracts --pharmacology
dc.subject.meshPlant Roots --chemistry
dc.subject.meshRats
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshStomach Ulcer --drug therapy
dc.titleAnti-ulcer & antioxidant activities of Hedranthera barteri {(Hook F.) Pichon} with possible involvement of H+, K+ ATPase inhibitory activity.en_US
dc.typeArticleen_US
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