Chandipura virus growth kinetics in vertebrate cell lines, insect cell lines & embryonated eggs.

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dc.contributor.authorJadi, R S
dc.contributor.authorSudeep, A B
dc.contributor.authorKumar, Satyendra
dc.contributor.authorArankalle, V A
dc.contributor.authorMishra, A C
dc.date.accessioned2011-11-30T06:32:29Z
dc.date.available2011-11-30T06:32:29Z
dc.date.issued2010-08
dc.description.abstractBackground & objectives: Since not much information on Chandipura virus is available, an attempt was made to study the growth kinetics of the virus in certain vertebrate, invertebrate cell lines and embryonated chicken eggs. Methods: Comparative study of Chandipura virus (CHPV) growth kinetics in three vertebrate cell lines [Vero E6, Rhabdo myosarcoma (RD), Porcine stable kidney (PS) cell lines], two insect cell lines [Aedes aegypti (AA) and Phlebotomus papatasi (PP-9) cell lines] and embryonated pathogen free chicken eggs was conducted, by tissue culture infective dose 50 per cent (TCID50) and indirect immunofluorescence assay (IFA). Results: All the cell lines and embryonated egg supported the growth of CHPV and yielded high virus titre. The vertebrate cell lines showed distinct cytopathic effect (CPE) within 4-6 h post infection (PI), while no CPE was observed in insect cell lines. PP-9 cell line was the most sensitive system to CHPV as viral antigen could be detected at 1 h PI by IFA. Interpretation & conclusions: Our results demonstrated that all the systems were susceptible to CHPV and achieved high yield of virus. However, the PP-9 cell line had an edge over the others due to its high sensitivity to the virus which might be useful for detection and isolation of the virus during epidemics.en_US
dc.identifier.citationJadi R S, Sudeep A B, Kumar Satyendra, Arankalle V A, Mishra A C. Chandipura virus growth kinetics in vertebrate cell lines, insect cell lines & embryonated eggs. Indian Journal of Medical Research. 2010 Aug; 132(2): 155-159.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135559
dc.language.isoenen_US
dc.source.urihttps://icmr.nic.in/ijmr/2010/august/0805.pdfen_US
dc.subjectAedes aegypti cell lineen_US
dc.subjectChandipura virusen_US
dc.subjectgrowth kineticsen_US
dc.subjectPhlebotomus papatasi cell lineen_US
dc.subjectVero E6 cell lineen_US
dc.subject.meshAedes
dc.subject.meshAnimals
dc.subject.meshCell Line, Tumor
dc.subject.meshCercopithecus aethiops
dc.subject.meshChickens
dc.subject.meshCulture Media --chemistry
dc.subject.meshFluorescent Antibody Technique, Indirect
dc.subject.meshKinetics
dc.subject.meshPhlebotomus
dc.subject.meshSus scrofa
dc.subject.meshTime Factors
dc.subject.meshVero Cells
dc.subject.meshVesiculovirus --growth & development
dc.titleChandipura virus growth kinetics in vertebrate cell lines, insect cell lines & embryonated eggs.en_US
dc.typeArticleen_US
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