Long Term Effect of Dental Unit Waterline Decontamination by Combining Sodium Hypochlorite with Chlorhexidine Gluconate of ICX™
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Date
2010-03-25
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Journal of the Dental Association of Thailand
Abstract
The purpose of this study was to observe the long term effect of dental unit waterline (DUWL) decontamination by combining shock treatment with Sodium hypochlorite (NaOCI) followed by continuous treatment with Chlorhexidine gluconate (Chlx) or ICM™ for 2 months. Twelve dental units were treated every 2 weeks with 0.005% NaOCI as shock treatment and divided into 3 groups. The first and the second groups (4 units each) were then treated continuously with 0.005% Chlx, or ICX™, respectively. The other group (4 units) was kept as control. Water from each DUWL was DUWL was sampled to observe total number of recoverable aerobic bacteria (CFUs). Data were analyzed by the Mann-Whitney U test. After shock treatment every 2 weeks for 2 months, the average CFUs in control group were reduced only for a short period and rapidly increased after each treatment. Continuous treatment with Chlx was slightly more effective than shock treatment alone but could not maintain the CFUs below that of ADA standard level of no more than 200 CFUs/ml. Continuous treatment with ICX™ significantly reduced CFUs to lower than standard level until day 9 after shock treatment. CFUs gradually increased to higher than standard level at the end of the 2nd week. Average CFUs from each single day after shock treatment in every cycle indicate a significant reduction in recoverable numbers of bacteria in Chlx and ICX™ group compared to control group. In conclusion, these data indicate that combining shock trratment with 0.005% NaOCI and continuous treatment with ICX™ was more effective in controlling DUWL contamination than shock treatment alone or in combination with Chlx.
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Journal of the Dental Association of Thailand; Vol.59 No.2 April-June 2009; 109-117