Added inositol regulates invertase secretion and glucose-repressed SUC2 gene expression in Saccharomyces sp. W4.

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dc.contributor.authorChi, Zhenmingen_US
dc.contributor.authorMa, Liyanen_US
dc.contributor.authorGao, Lingmeien_US
dc.contributor.authorDuan, Xiaohuien_US
dc.date.accessioned2007-06-27en_US
dc.date.accessioned2009-05-27T09:45:48Z
dc.date.available2007-06-27en_US
dc.date.available2009-05-27T09:45:48Z
dc.date.issued2007-06-27en_US
dc.description.abstractThe effect of inositol supplementation on glucose derepression, invertase secretion and SUC2 gene expression in Saccharomyces sp. W4 was studied. Invertase secretion was repressed, when the yeast cells, grown the synthetic medium without inositol (I(-) medium) contained more than 0.2% (w/v) initial concentration of glucose. However, in the same medium plus inositol (I(+) medium, inositol conc. 100 microg/100 ml), invertase secretion was repressed only at glucose concentrations higher than 2.0% (w/v). Results showed that secreted invertase activity increased only in the I+ medium, whereas intracellular invertase activity remained constant in both media during the cell, growth. The mRNA encoding secreted invertase was higher in the glucose-derepressed cells grown in the I(+) medium than in the glucose-repressed cells grown in the I(-) medium. Similarly, phosphatidylinositol (PI) content was significantly higher in the cells grown in the I(+) medium than in the I(-) medium. These results indicated that PI might be involved in the glucose derepression, invertase secretion and SUC2 gene expression at the transcriptional level in the yeast.en_US
dc.description.affiliationUNESCO Chinese Center of Marine Biotechnology, Ocean University of China, Yushan Road, No. 5, Qingdao, China. zhenming@sdu.edu.cnen_US
dc.identifier.citationChi Z, Ma L, Gao L, Duan X. Added inositol regulates invertase secretion and glucose-repressed SUC2 gene expression in Saccharomyces sp. W4. Indian Journal of Biochemistry & Biophysics. 2007 Jun; 44(3): 152-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28486
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshCell Culture Techniquesen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshGene Expression Regulation, Fungalen_US
dc.subject.meshGlucose --metabolismen_US
dc.subject.meshInositol --metabolismen_US
dc.subject.meshPhospholipids --metabolismen_US
dc.subject.meshRNA --metabolismen_US
dc.subject.meshRNA, Fungal --geneticsen_US
dc.subject.meshRNA, Messenger --metabolismen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshSaccharomyces --metabolismen_US
dc.subject.meshSaccharomyces cerevisiae Proteins --biosynthesisen_US
dc.subject.meshTime Factorsen_US
dc.subject.meshbeta-Fructofuranosidase --biosynthesisen_US
dc.titleAdded inositol regulates invertase secretion and glucose-repressed SUC2 gene expression in Saccharomyces sp. W4.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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