Purification and characterization of ribulose-1,5-bisphosphate carboxylase from triticale.

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dc.contributor.authorKhan, M Aen_US
dc.contributor.authorDixit, Aen_US
dc.contributor.authorUpadhyaya, K Cen_US
dc.date.accessioned1994-04-01en_US
dc.date.accessioned2009-05-27T09:31:59Z
dc.date.available1994-04-01en_US
dc.date.available2009-05-27T09:31:59Z
dc.date.issued1994-04-01en_US
dc.description.abstractRibulose-1,5-bisphosphate carboxylase has been isolated from a synthetic cereal triticale and purified using a newly developed rapid procedure involving precipitation with ammonium sulphate (35-55% saturation), DEAE-cellulose (DE-52) chromatography and filtration through Sepharose CL-68. Molecular weights of the enzyme subunits are 15.5 and 52 kDa which corresponds to 540 kDa for the hexadecameric holoenzyme. Isoelectric focussing showed that the enzyme has a pI of 4.2. Various kinetic constants determined under aerobic conditions are: Km (CO2), 118 microM; Km (RuBP), 220 microM (at 20 mM NaHCO3) and Vmax, 690 nmole CO2 fixed/mg enzyme/min.en_US
dc.description.affiliationSchool of Life Sciences, Jawaharlal Nehru University, New Delhi.en_US
dc.identifier.citationKhan MA, Dixit A, Upadhyaya KC. Purification and characterization of ribulose-1,5-bisphosphate carboxylase from triticale. Indian Journal of Biochemistry & Biophysics. 1994 Apr; 31(2): 121-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27792
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshCationsen_US
dc.subject.meshCereals --enzymologyen_US
dc.subject.meshChromatography, Gelen_US
dc.subject.meshElectrophoresis, Polyacrylamide Gelen_US
dc.subject.meshEnzyme Activationen_US
dc.subject.meshKineticsen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshRibulose-Bisphosphate Carboxylase --chemistryen_US
dc.subject.meshSalts --pharmacologyen_US
dc.titlePurification and characterization of ribulose-1,5-bisphosphate carboxylase from triticale.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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