Analysis of Japanese encephalitis (JE) virus genome and implications for recombinant JE vaccine.

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dc.contributor.authorYasui, Ken_US
dc.contributor.authorMiyamoto, Men_US
dc.contributor.authorKimura-Kuroda, Jen_US
dc.contributor.authorYasuda, Aen_US
dc.contributor.authorMatsuura, Yen_US
dc.contributor.authorSato, Ten_US
dc.contributor.authorKojima, Aen_US
dc.contributor.authorKubonoya, Hen_US
dc.date.accessioned2009-05-27T16:48:25Z
dc.date.available2009-05-27T16:48:25Z
dc.date.issued1990-12-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractFrom the information of nucleotide sequences and deduced amino acid sequences of flaviviruses including JEV, we can postulate processing mechanisms of a polyprotein translated from single long open reading frame of the genome and mechanisms of construction of antigenic structures of structural proteins with biologically active forms after these proteins are translated. The results of comparative analysis of amino acid sequences among flaviviruses and epitope analysis on the E proteins which are the most important antigens for protective immunity suggest that the E protein of flaviviruses may have a similar structure closely related to each other. PrM and E proteins which had predictable signal sequences upstream on the N terminals were expressed with antigenically active form and molecular size the same as the authentic ones by the recombinant viruses. However, the recombinant viruses which had no such signal sequence expressed unprocessed proteins with antigenically denatured forms. These results suggest that normal proteolytic processing is needed to construct biologically active structures of JEV structural proteins. The E proteins which were expressed by the recombinant viruses as antigenically active form could elicit nutralizing and HI antibodies in animals and protective immunity in mice. The recombinant vaccinia viruses which express the E protein could induce strong immunologic memory against the E protein in mice. These results indicate that the development of a new type of vaccine against JEV will become possible in future.en_US
dc.description.affiliationDepartment of Microbiology, Tokyo Metropolitan Institute for Neurosciences, Japan.en_US
dc.identifier.citationYasui K, Miyamoto M, Kimura-Kuroda J, Yasuda A, Matsuura Y, Sato T, Kojima A, Kubonoya H. Analysis of Japanese encephalitis (JE) virus genome and implications for recombinant JE vaccine. The Southeast Asian Journal of Tropical Medicine and Public Health. 1990 Dec; 21(4): 663-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/36053
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshAmino Acid Sequence --geneticsen_US
dc.subject.meshCross Reactionsen_US
dc.subject.meshEncephalitis, Japanese --geneticsen_US
dc.subject.meshEpitopesen_US
dc.subject.meshGene Expression --geneticsen_US
dc.subject.meshGenes, Viral --geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshProteins --geneticsen_US
dc.subject.meshVaccines, Synthetic --geneticsen_US
dc.titleAnalysis of Japanese encephalitis (JE) virus genome and implications for recombinant JE vaccine.en_US
dc.typeJournal Articleen_US
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