Assessment of HER-2/neu status in breast cancer using fluorescence in situ hybridization & immunohistochemistry: Experience of a tertiary cancer referral centre in India.

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dc.contributor.authorPanjwani, Poonam
dc.contributor.authorEpari, Sridhar
dc.contributor.authorKarpate, Arti
dc.contributor.authorShirsat, Hemlata
dc.contributor.authorRajsekharan, Preetha
dc.contributor.authorBasak, Ranjan
dc.contributor.authorShet, Tanuja
dc.contributor.authorChinoy, Roshni
dc.contributor.authorChacko, Roy
dc.contributor.authorGursale, Sampada
dc.contributor.authorBaraskar, Nayana
dc.contributor.authorGupta, Sudeep
dc.contributor.authorHawaldar, Rohini
dc.contributor.authorDesai, Sangeeta
dc.date.accessioned2011-12-01T05:07:40Z
dc.date.available2011-12-01T05:07:40Z
dc.date.issued2010-09
dc.description.abstractBackground & objective: Determination of HER2 status in breast cancer has become important to identify potential candidates for anti-HER2 therapy. In this study we compared fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) for the determination of HER2 status in breast cancer patients referred to a tertiary care referral centre. Methods: A total of 200 cases of invasive breast cancer were evaluated for HER2 status using IHC and FISH and results were compared. Results: The IHC 3+ (93.9%) and IHC negative (85.9%) cases showed good concordance with the corresponding FISH results; while 66.6 per cent of IHC 2+ cases showed gene amplification by FISH. In addition, hormone receptor expression and HER2 gene status showed a statistically significant inverse association (P<0.05). Interpretation & conclusion: These findings reaffirm IHC as a prudent first-step to screen tissue samples for HER2 status and to determine suitability for technically demanding FISH test and the dual coloured FISH as a gold standard for determination of HER2/neu status in IHC equivocal cases of breast carcinoma.en_US
dc.identifier.citationPanjwani Poonam, Epari Sridhar, Karpate Arti, Shirsat Hemlata, Rajsekharan Preetha, Basak Ranjan, Shet Tanuja, Chinoy Roshni, Chacko Roy, Gursale Sampada, Baraskar Nayana, Gupta Sudeep, Hawaldar Rohini, Desai Sangeeta. Assessment of HER-2/neu status in breast cancer using fluorescence in situ hybridization & immunohistochemistry: Experience of a tertiary cancer referral centre in India. Indian Journal of Medical Research. 2010 Sept; 132(3): 287-294.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135582
dc.language.isoenen_US
dc.source.urihttps://icmr.nic.in/ijmr/2010/september/0909.pdfen_US
dc.subjectBreast canceren_US
dc.subjectfluorescent in situ hybridization (FISH)en_US
dc.subjectHER2/neuen_US
dc.subjectimmunohistochemistry (IHC)en_US
dc.subjecttrastuzumaben_US
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshBiological Markers --analysis
dc.subject.meshBreast Neoplasms --diagnosis
dc.subject.meshBreast Neoplasms --pathology
dc.subject.meshfemale
dc.subject.meshHumans
dc.subject.meshImmunoenzyme Techniques --methods
dc.subject.meshIn Situ Hybridization, Fluorescence --methods
dc.subject.meshIndia
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshReceptor, erbB-2 --analysis
dc.subject.meshReceptor, erbB-2 --genetics
dc.subject.meshSensitivity and Specificity
dc.titleAssessment of HER-2/neu status in breast cancer using fluorescence in situ hybridization & immunohistochemistry: Experience of a tertiary cancer referral centre in India.en_US
dc.typeArticleen_US
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