High performance liquid chromatographic determination of cox-2 inhibitor rofecoxib in human plasma.

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dc.contributor.authorMandal, Uen_US
dc.contributor.authorGanesan, Men_US
dc.contributor.authorJayakumar, Men_US
dc.contributor.authorPal, T Ken_US
dc.contributor.authorChattaraj, T Ken_US
dc.contributor.authorRay, Krishnangshuen_US
dc.contributor.authorBanerjee, S Nen_US
dc.date.accessioned2003-08-10en_US
dc.date.accessioned2009-05-31T15:45:25Z
dc.date.available2003-08-10en_US
dc.date.available2009-05-31T15:45:25Z
dc.date.issued2003-08-10en_US
dc.description.abstractA convenient, sensitive and simple method for the determination of rofecoxib in human plasma is presented. The analytical technique is based on reversed phase high performance liquid chromatography coupled with UV detector (Knauer, Germany) set at 272 nm. The retention time of rofecoxib after recovery from plasma, was 8.9 minutes. The method has been validated over a linear range of 50-450 ng/ml from plasma. After validation the method was used to study the pharmacokinetic profile of rofecoxib in 6 healthy volunteers as per DCGI guidelines after administration of a single oral dose (50 mg). The extraction efficiency from plasma varied from 93.95-99.58%. The minimum quantifiable concentration was set at 50 ng/ml (% CV < 10%). The pharmacokinetic parameters were Cmax = 318.58 +/- 30.65 ng/ml at tmax = 2.66 +/- 0.25 hours, AUC0-t = 4007.88 +/- 438.32 ng hour/ml, AUC0-yen = 5454.66 +/- 822.29 ng hour/ml, Kel = 0.0433 +/- 0.0067/hour, and t1/2 = 16.36 +/- 2.89 hours.en_US
dc.description.affiliationBioequivalence Study Centre, Department of Pharmaceutical Technology, Jadavpur University, Kolkata 700032.en_US
dc.identifier.citationMandal U, Ganesan M, Jayakumar M, Pal TK, Chattaraj TK, Ray K, Banerjee SN. High performance liquid chromatographic determination of cox-2 inhibitor rofecoxib in human plasma. Journal of the Indian Medical Association. 2003 Aug; 101(8): 486-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/103759
dc.language.isoengen_US
dc.source.urihttps://www.jimaonline.org.in/en_US
dc.subject.meshArea Under Curveen_US
dc.subject.meshChromatography, High Pressure Liquiden_US
dc.subject.meshCyclooxygenase Inhibitors --blooden_US
dc.subject.meshHumansen_US
dc.subject.meshLactones --blooden_US
dc.subject.meshReference Standardsen_US
dc.subject.meshReproducibility of Resultsen_US
dc.subject.meshSulfonesen_US
dc.titleHigh performance liquid chromatographic determination of cox-2 inhibitor rofecoxib in human plasma.en_US
dc.typeJournal Articleen_US
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