Immobilization of amyloglucosidase.

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dc.contributor.authorShankar, Ven_US
dc.contributor.authorNehete, P Nen_US
dc.contributor.authorKothari, R Men_US
dc.date.accessioned1993-02-01en_US
dc.date.accessioned2009-05-27T09:41:01Z
dc.date.available1993-02-01en_US
dc.date.available2009-05-27T09:41:01Z
dc.date.issued1993-02-01en_US
dc.description88 references.en_US
dc.description.abstractEnzymatic hydrolysis of starch for the production of glucose syrups of various compositions has assumed considerable commercial significance due to the extensive application of these syrups in food and beverage industries. Hydrolysis of starch to glucose involves liquefaction of the gelatinized starch with acid or thermostable alpha-amylase followed by saccharification to glucose by amyloglucosidase. Large scale saccharification of liquefied starch to glucose using soluble enzyme is time consuming and requires 48-72 hr at pH 4.5 and 55-60 degrees C. Since, by replacing soluble amyloglucosidase with immobilized enzyme, it is possible to reduce the conversion time, several methods have been tried to obtain a highly active and stable immobilized preparation capable of converting high concentrations of liquefied starch to glucose. However, till today, immobilized amyloglucosidase has not found industrial application as no immobilized system has shown high temperature stability and conversion efficiency comparable to that of the soluble enzyme.en_US
dc.description.affiliationIndustrial Enzyme Division, Sarabhai Research Centre, Baroda.en_US
dc.identifier.citationShankar V, Nehete PN, Kothari RM. Immobilization of amyloglucosidase. Indian Journal of Biochemistry & Biophysics. 1993 Feb; 30(1): 62-70en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/28247
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshEnzymes, Immobilized --isolation & purificationen_US
dc.subject.meshFood Technologyen_US
dc.subject.meshGlucan 1,4-alpha-Glucosidase --isolation & purificationen_US
dc.subject.meshStarch --chemistryen_US
dc.subject.meshSurface Propertiesen_US
dc.titleImmobilization of amyloglucosidase.en_US
dc.typeJournal Articleen_US
dc.typeReviewen_US
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