Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin M

Simple item page
dc.contributor.authorJain, Amitaen_US
dc.contributor.authorJain, Parulen_US
dc.contributor.authorRebello, Sanjay C.en_US
dc.contributor.authorTodaria, Moulikaen_US
dc.contributor.authorKaur, Harmanmeeten_US
dc.contributor.authorGupta, Niveditaen_US
dc.contributor.authorAggarwal, Neerajen_US
dc.contributor.authorVijay, Neetuen_US
dc.contributor.authorNarayan, Jitendraen_US
dc.date.accessioned2023-08-19T04:51:51Z
dc.date.available2023-08-19T04:51:51Z
dc.date.issued2023-06
dc.description.abstractBackground & objectives: The diagnosis of scrub typhus (ST) is usually done using enzyme-linked immunosorbent assay (ELISA) due to its ease of performance and reading objectivity. The cut-off value for ELISA needs to be calculated for each geographical location as it depends on zonal endemicity of the disease. This study was, therefore, undertaken to calculate the pan-India cut-off for anti-Orientia tsutsugamushi (OT) immunoglobulin M (IgM) by ELISA. Methods: Samples from cases (cases of ST) and controls (voluntary, consenting, healthy adults) were collected by a network of 29 laboratories across India and tested for anti-OT IgM by immunofluorescence assay (IFA), the considered gold standard test. These samples were retested by ELISA for anti-OT IgM and their optical densities (ODs) were used for cut-off estimation by receiver operating characteristic (ROC) curve. Results: Anti-OT IgM ELISA ODs from 273 controls and 136 cases were used for the cut-off estimation. The ODs of the anti-OT IgM ELISA on healthy individuals and those of confirmed ST cases ranged from 0.1 to 0.75 and 0.5 to 4.718, respectively. ROC curve-based cut-off for ELISA was calculated as 0.554 at a sensitivity of 95.2 per cent and specificity of 95.1 per cent. A value of >1 was noted to have a specificity of 100 per cent in diagnosing ST. Interpretation & conclusions: The cut-off calculated for India was similar to the previous cut-off that was used until now.en_US
dc.identifier.affiliationsDepartment of Microbiology, King George’s Medical University, Lucknow, Uttar Pradeshen_US
dc.identifier.affiliationsDivision of Epidemiology and Communicable Diseases, Indian Council of Medical Researchen_US
dc.identifier.affiliationsDepartment of Health Research, Government of India, New Delhi, Indiaen_US
dc.identifier.citationJain Amita, Jain Parul, Rebello Sanjay C., Todaria Moulika, Kaur Harmanmeet, Gupta Nivedita, Aggarwal Neeraj, Vijay Neetu, Narayan Jitendra. Determination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin M . Indian Journal of Medical Research. 2023 Jun; 157(6): 519-523en_US
dc.identifier.issn0971-5916
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/223557
dc.languageenen_US
dc.publisherWolters Kluwer – Medknowen_US
dc.relation.issuenumber6en_US
dc.relation.volume157en_US
dc.source.urihttps://doi.org/10.4103/ijmr.IJMR_4098_20en_US
dc.subjectEnzyme-linked immunosorbent assay cut-offen_US
dc.subjectindirect immunofluorescence assayen_US
dc.subjectOrientia tsutsugamushien_US
dc.subjectimmunoglobulin Men_US
dc.subjectscrub typhusen_US
dc.titleDetermination of a cut-off value for the serological diagnosis of scrub typhus by detecting anti-Orientia tsutsugamushi immunoglobulin Men_US
dc.typeJournal Articleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
IJMR2023v157n6p519.pdf
Size:
1.18 MB
Format:
Adobe Portable Document Format
Download
Collections
Indian Journal of Medical Research