Use of polymerase chain reaction (PCR) for detection of Chlamydia trachomatis infection in cervical swab samples.

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dc.contributor.authorMania-Pramanik, Jen_US
dc.contributor.authorDonde, U Men_US
dc.contributor.authorMaitra, Aen_US
dc.date.accessioned2009-05-28T08:12:52Z
dc.date.available2009-05-28T08:12:52Z
dc.date.issued2001-09-01en_US
dc.description.abstractA polymerase chain reaction (PCR) based method has been set up for detection of Chlamydia trachomatis (C. trachomatis) infection in single cervical swab samples. A primer pair specific to the major outer membrane protein (MOMP) gene common to all serotypes of C. trachomatis was used. This method was validated for its sensitivity as well as specificity. A minimum Ing of DNA could be used for detection of the infection. Specificity of the method was confirmed by carrying out a sample dilution curve. The cervical swab samples analysed in the present study were in coded form for validation of the PCR with an established commercial ELISA (Chlamydiazyme). Both the sensitivity and specificity of PCR was 100% when the ELISA results of these samples were decoded. Thus, this PCR technique could be used for better diagnosis of C. trachomatis infection in comparison to the commercially available ELISA technique.en_US
dc.description.affiliationInstitute for Research in Reproduction (ICMR), Jehangir Merwanji Street, Parel, Mumbai-400 012, India.en_US
dc.identifier.citationMania-Pramanik J, Donde UM, Maitra A. Use of polymerase chain reaction (PCR) for detection of Chlamydia trachomatis infection in cervical swab samples. Indian Journal of Dermatology, Venereology and Leprology. 2001 Sep-Oct; 67(5): 246-50en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/52277
dc.language.isoengen_US
dc.source.urihttps://www.ijdvl.comen_US
dc.titleUse of polymerase chain reaction (PCR) for detection of Chlamydia trachomatis infection in cervical swab samples.en_US
dc.typeJournal Articleen_US
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