Evaluation on the Effect of Ganoderma lucidum (Leyss.ex Fr) Karst. Extracts on p53, Bcl-2 and Bax mRNA Expression on Cultured Mammalian Cells

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dc.contributor.authorJarunya Ngamkhamen_US
dc.contributor.authorMati Rienkitjakarnen_US
dc.contributor.authorPorntipa Pichaen_US
dc.date.accessioned2011-02-22T08:15:48Z
dc.date.available2011-02-22T08:15:48Z
dc.date.created2010-04-07en_US
dc.date.issued2010-04-07en_US
dc.description.abstractGanoderma lucidum is a well-known traditional Chinese medicine herb and widely used as an herbal medicine for promoting vitality and longevity in China and other Asian countries. It has also been used for the prevention or treatment of a variety of diseases including cancer. In this study, we investigated the effect of ethanol and water extracts from G. lucidum underlying anticancer activities and apoptosis-related gene expression on cultured mammalian cancer cell lines. The anticancer activities were undertaken with total cell protein determination method on two cancer cell lines, human mammary carcinoma; MCF-7 and human cervical carcinoma; HeLa. The results were expressed by median effective dose (ED₅₀). The apoptosis-related gene expression was analyzed by using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) technique on MCF-7 and HeLa cells. The relative intensity of PCR product from p53, Bcl-2 and Bax gene were quantified and normalized with house-keeping gene; \â-actin.From our investigation, the ethanol and water extracts showed the proliferation inhibitory effect on cultured mammalian cancer cells. The ethanol extract exhibited ED₅₀ values at 208.83\±7.28 \μg/ml and 303.61\±5.11 \μg/ml on MCF-7 and HeLa cell, respectively, while the extracts from water exerted ED₅₀ values at 438.69\±14.31 \μg/ml and 671.43\±3.37 \μg/ml on MCF-7 and HeLa, respectively. Both extracts from G. lucidum reduced cell viabilities in a dose-dependent manner on three cancer cell lines. The results from semi-quantitative RT-PCR assay indicated that the expression of p53 and Bax mRNA in MCF-7 and HeLa were increased, whereas the expression of Bcl-2 mRNA was slightly decreased. Our findings demonstrated that the ethanol and water extracts from G. lucidum slightly exhibited anticancer activities and induced the apoptosis-related gene expression. Therefore, further investigation on cellular and molecular levels and also in the animal tumor model is promising. In addition, further studies on preclinical and clinical with G. lucidum are recommended to validate this herbal medicine for prevention and treatment of cancer.en_US
dc.identifier.citationThai Cancer Journal; Vol.29 No.1 January-March 2009; 13-24en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/132353
dc.language.isoen_USen_US
dc.publisherThai Cancer Journalen_US
dc.rightsNational Cancer Institute of Thailand, Bangkok, Thailanden_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/TCJ/issue/archiveen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/TCJ/article/view/3058en_US
dc.titleEvaluation on the Effect of Ganoderma lucidum (Leyss.ex Fr) Karst. Extracts on p53, Bcl-2 and Bax mRNA Expression on Cultured Mammalian Cellsen_US
dc.typeResearch Articlesen_US
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