Preservation of Leishmania donovani promastigotes in blood agar slants.

Simple item page
dc.contributor.authorMuniaraj, Men_US
dc.contributor.authorGupta, A Ken_US
dc.contributor.authorNarayan, Sen_US
dc.contributor.authorSingh, Den_US
dc.contributor.authorSinha, P Ken_US
dc.contributor.authorKishore, Ken_US
dc.contributor.authorDas, Pen_US
dc.contributor.authorBhattacharya, S Ken_US
dc.date.accessioned2005-09-04en_US
dc.date.accessioned2009-06-01T19:02:25Z
dc.date.available2005-09-04en_US
dc.date.available2009-06-01T19:02:25Z
dc.date.issued2005-09-04en_US
dc.description.abstractLong-term cultivation of Leishmania promastigotes by weekly passage to fresh medium was reported to be disadvantageous because needs labor, risk of contamination, lowering in infectivity and virulence pattern. Cryopreservation and Lyophilization require expensive facilities which could be a burden and unaffordable to most laboratories of developing countries where the disease is endemic. These problems could be minimized by simple preservation of Leishmania donovani promastigotes in blood agar slants at 7-8 degrees C for 6-7 months. The preserved promastigotes were examined for viability up to one year at a regular interval of one month. Viable promastigotes were found and revived successfully from all the slants stored up to 7 months after that, the viability of promastigotes was found to be decreased in the slants of 8-9 month storage. No viable promastigotes were recovered from the slants stored up to 11-12 months. By this method, the promastigotes can easily be stored up to 7 months without loss of biological activity. The number of passage of promastigotes to fresh medium has been greatly reduced by this method from 30 times to 01 when compared with weekly passage in liquid medium. This simple and economical method can be recommended for short storage of Leishmania culture without loss of any activity.en_US
dc.description.affiliationRajendra Memorial Research Institute of Medical Sciences, (ICMR) Agam Kuan, Patna.en_US
dc.identifier.citationMuniaraj M, Gupta AK, Narayan S, Singh D, Sinha PK, Kishore K, Das P, Bhattacharya SK. Preservation of Leishmania donovani promastigotes in blood agar slants. Journal of Communicable Diseases. 2005 Sep; 37(3): 191-5en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/112663
dc.language.isoengen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlooden_US
dc.subject.meshCell Culture Techniques --methodsen_US
dc.subject.meshCryopreservation --methodsen_US
dc.subject.meshCulture Mediaen_US
dc.subject.meshFreeze Drying --methodsen_US
dc.subject.meshHumansen_US
dc.subject.meshLeishmania donovani --growth & developmenten_US
dc.subject.meshLeishmaniasis --diagnosisen_US
dc.subject.meshSpecimen Handlingen_US
dc.titlePreservation of Leishmania donovani promastigotes in blood agar slants.en_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Journal of Communicable Diseases