Design and evaluation of liposomal delivery system for L-Asparaginese.

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dc.contributor.authorDe, Anindita
dc.contributor.authorVenkatesh, D Nagaswamy
dc.date.accessioned2014-05-26T12:20:47Z
dc.date.available2014-05-26T12:20:47Z
dc.date.issued2012-08
dc.description.abstractDespite of its effective anti-tumour activity,L-Asparaginase has limited clinical application due to the high rate of clinical hypersensitivity. In an attempt to develop a liposomal drug delivery for L-Asparaginase, enzyme loaded liposomes were formulated using soy lecithin, cholesterol and charge inducers by thin film hydration method. The effect of various components of the liposomes including the concentration of lecithin and cholesterol with or without the charge inducers on the entrapment efficiency and short term invitro cytotoxicity study was systematically investigated. The average particle sizes of the vesicles were found to be 43.2, 35.6 and 65.8 μm respectively for neutral, positive and negative liposomes. The percentage of drug loading was found to be 1.95, 2.39 and 2.35 % respectively for neutral, positive and negative liposomes.The invitro release study of L-Asparaginase was carried out using normal saline as dissolution medium and the release was found to be 86.88, 78.29 and 82.04 % respectively for neutral, positive and negative liposomes. The release of LAsparaginase from liposomes was followed first order kinetics obeying non-Fickian diffusion. A short term cytotoxicity study was carried out using Ehrlich Ascites Carcinoma cells (EAC cells) which revealed that the cytotoxicity concentration CTC50 for pure drug was found to be 64 mcg as compared to liposomal formulation of 50 mcg.en_US
dc.identifier.citationDe Anindita, Venkatesh D Nagaswamy. Design and evaluation of liposomal delivery system for L-Asparaginese. Journal of Applied Pharmaceutical Science. 2012 Aug; 2(8): 112-117.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/151349
dc.language.isoenen_US
dc.source.urihttps://www.japsonline.com/counter.php?aid=600en_US
dc.subjectL-Asparaginaseen_US
dc.subjectliposomesen_US
dc.subjectcharge inducersen_US
dc.subjectrelease kineticsen_US
dc.subjectin vitro cytotoxicity studiesen_US
dc.titleDesign and evaluation of liposomal delivery system for L-Asparaginese.en_US
dc.typeArticleen_US
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