Rapid diagnosis of vaginal carriage of group B beta haemolytic streptococcus by an enrichment cum antigen detection test.

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dc.contributor.authorDas, Aninditaen_US
dc.contributor.authorRay, Pallaben_US
dc.contributor.authorSharma, Meeraen_US
dc.contributor.authorGopalan, Saralaen_US
dc.date.accessioned2003-06-30en_US
dc.date.accessioned2009-05-27T07:27:27Z
dc.date.available2003-06-30en_US
dc.date.available2009-05-27T07:27:27Z
dc.date.issued2003-06-30en_US
dc.description.abstractBACKGROUND & OBJECTIVES: Group B beta haemolytic streptococcus (GBS) is a frequent colonizer of the maternal genital tract causing peripartum fever, puerperal sepsis, neonatal sepsis and neonatal meningitis. The conventional methods for detection of maternal colonization take 24-48 h. We made an attempt to standardize a rapid enrichment cum antigen detection test to screen pregnant women for GBS colonization in less than 8 h, so as to enable early institution of measures to prevent neonatal sepsis. METHODS: Vaginal swabs of 100 women >36 wk of gestation were inoculated onto enrichment broth (Todd Hewitt broth with lysed horse blood and antibiotics). After incubation for 1,2,4,6, and 18 h, the broth was cultured on sheep blood agar. In culture positive cases, the enrichment broth was subjected to antigen detection by latex agglutination test (LAT). For further evaluation of the rapid test, another group of 100 pregnant women were screened for GBS carriage by 6 h enrichment broth culture followed by antigen detection test. RESULTS: Five of the first group yielded GBS on culture and all were positive for GBS antigen after 6 h enrichment. Thirteen of the second group were positive for the antigen, but GBS could be isolated in ten only. This enrichment cum antigen detection test showed sensitivity, specificity, and positive and negative predictive values of 100, 98.4, 83.3 and 100 per cent respectively and could detect as few as 10(3) cfu/ml organisms. Maternal vaginal carriage of GBS was 7.5 per cent (15/200). INTERPRETATION & CONCLUSION: Six hours of enrichment followed by antigen detection proved to be a rapid and reliable method for detection of GBS colonization. This test is easy to perform making it an ideal test for screening GBS vaginal colonization at labour and starting chemoprophylaxis, where indicated on the same day, before the woman is discharged.en_US
dc.description.affiliationDepartment of Medical Microbiology, Postgraduate Institute of Medical Education & Research, Chandigarh, India.en_US
dc.identifier.citationDas A, Ray P, Sharma M, Gopalan S. Rapid diagnosis of vaginal carriage of group B beta haemolytic streptococcus by an enrichment cum antigen detection test. Indian Journal of Medical Research. 2003 Jun; 117(): 247-52en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/20784
dc.language.isoengen_US
dc.source.urihttps://icmr.nic.in/ijmr/ijmr.htmen_US
dc.subject.meshAdulten_US
dc.subject.meshAntigens, Bacterial --analysisen_US
dc.subject.meshBacteriological Techniques --statistics & numerical dataen_US
dc.subject.meshCarrier State --diagnosisen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshInfant, Newbornen_US
dc.subject.meshPregnancyen_US
dc.subject.meshSensitivity and Specificityen_US
dc.subject.meshSepsis --prevention & controlen_US
dc.subject.meshStreptococcal Infections --diagnosisen_US
dc.subject.meshStreptococcus agalactiae --immunologyen_US
dc.subject.meshVagina --microbiologyen_US
dc.titleRapid diagnosis of vaginal carriage of group B beta haemolytic streptococcus by an enrichment cum antigen detection test.en_US
dc.typeJournal Articleen_US
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