Effect of phospholipase-D on rat kidney mitochondria.
| dc.contributor.author | Zaidi, S N A | |
| dc.contributor.author | Shipstone, A C | |
| dc.contributor.author | Garg, N K | |
| dc.date.accessioned | 2015-07-17T07:10:06Z | |
| dc.date.available | 2015-07-17T07:10:06Z | |
| dc.date.issued | 1979-03 | |
| dc.description.abstract | Incubation of purified rat kidney mitochondrial fraction with phospholipase- D resulted in the accumulation of phosphatidic acid in the membrane due to the degradation of membrane-bound phosphatidylcholine, -serine and -ethanolamine Simultaneously with the hydrolysis of the phospholipids, cholesterol and protein were released from the mitochondrial membrane into the medium, and binding of Ca2+ by mitochondrial membranes increased. Phospholipase Dtreated mitochondrial fraction exhibited increased swelling in vitro in the early stages of incubation (15 min) after which the mitochondria were ruptured. Membrane- bound adenosine triphosphatase was partially inactivated and the enzyme activity was not significantly restored by incubation with sonicated dispersions of phosphatidylcholine, -serine and cholesterol. These results indicate that removal of choline, serine and ethanolamine from membrane-bound phospholipids disrupt phospholipid-cholesterol and phospholipid-protein association and affect functions of the membrane. | en_US |
| dc.identifier.citation | Zaidi S N A, Shipstone A C, Garg N K. Effect of phospholipase-D on rat kidney mitochondria. Journal of Biosciences. 1979 Mar; 1(1): 75-82. | en_US |
| dc.identifier.uri | https://imsear.searo.who.int/handle/123456789/159932 | |
| dc.language.iso | en | en_US |
| dc.source.uri | https://www.ias.ac.in/jarch/jbiosci/1/75-82.pdf | en_US |
| dc.subject | Mitochondria | en_US |
| dc.subject | phospholipase-D | en_US |
| dc.subject | phosphatidylcholine | en_US |
| dc.subject | phosphatidylethanolamine | en_US |
| dc.subject | phosphatidylserine | en_US |
| dc.subject | swelling | en_US |
| dc.subject | adenosine triphosphatase | en_US |
| dc.subject | Ca2+ binding | en_US |
| dc.title | Effect of phospholipase-D on rat kidney mitochondria. | en_US |
| dc.type | Article | en_US |