Screening of anticancer activity from Streptomyces spp.

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dc.contributor.authorWipada Ngearnthaworn; Division of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakorn Si Thammaraten_US
dc.contributor.authorSurapong Klybud; Division of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakorn Si Thammaraten_US
dc.contributor.authorSulaiman Awae; Division of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakorn Si Thammaraten_US
dc.contributor.authorPhuwadol Bangrak; Division of Biology, School of Science, Walailak Universityen_US
dc.contributor.authorWarangkana Chunglok; Utilization of Natural Product Unit, Walailak Universityen_US
dc.contributor.authorMonthon Lertcanawanichakul; Utilization of Natural Product Unit, Walailak Universityen_US
dc.date.accessioned2011-02-20T04:32:57Z
dc.date.available2011-02-20T04:32:57Z
dc.date.created2010-04-11en_US
dc.date.issued2010-04-11en_US
dc.description.abstractThe study was aimed to evaluate anticancer activity from Streptomyces spp. Human mouth carcinoma cell line (CLS-354 cell) was used as a cell model for cytotoxicity testing. Culture broth and whole cell lysates obtained from three isolates of Streptomyces spp. (CO4, WU6 and WU10) were evaluated for anticancer activity in comparison with 5-fluorouracil (5-FU), known as anticancer drug. Anticancer activity was markedly observed from lyophilized culture broth obtained from CO4 \> WU6 \> WU10. The half maximal inhibitory concentration (IC50) obtained from CO4, WU6, and WU10 was 5 mg protein/ml (5.88 mg/ml), 31 mg protein/ml (34.83 mg/ml), and 23 mg protein/ml (25.62 mg/ml), respectively. Whole cell lysates obtained from CO4 revealed IC50 of 78 mg protein/ml. The lysates from WU6 or WU10 revealed IC50 of more than 625 mg protein/ml. This was suggested that lyophilized culture broth obtained from Streptomyces spp. was more potent anticancer activity than did whole cell lysates. Anticancer drug, 5-FU was more efficient than lyophilized culture broth obtained from Streptomyces spp with IC50 of 0.75 mg/ml. This may depend on the purity of bioactive compounds produced from Streptomyces spp. Thus, Streptomyces spp. may provide promising sources of anticancer bioactive agents which need to be further evaluated.en_US
dc.identifier.citationJournal of Medical Technology and Physical Therapy; Volume 22 Number 1, January - April 2010; 37-44en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/130896
dc.language.isoen_USen_US
dc.publisherKhon Kaen Universityen_US
dc.rightsKhon Kaen Universityen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/issue/archiveen_US
dc.source.urihttps://thailand.digitaljournals.org/index.php/JMTPT/article/view/3378en_US
dc.titleScreening of anticancer activity from Streptomyces spp.en_US
dc.typeORIGINAL ARTICLEen_US
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