Xylene-free staining: Is it possible and practical?

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dc.contributor.authorYadav, Sen_US
dc.contributor.authorMallya, Ven_US
dc.contributor.authorKhurana, Nen_US
dc.date.accessioned2020-04-10T01:50:08Z
dc.date.available2020-04-10T01:50:08Z
dc.date.issued2019-06
dc.description.abstractGood paraffin sections are key to correct histopathological diagnosis. Xylene is hazardous to health, expensive, and difficult to dispose. Various substitutes have been tried without success. We aimed to examine if 1.7% dishwasher soap (DWS) aqueous solution and refined mineral oil (RMO) for deparaffinization can replace xylene. Fifty tissue blocks consisting of benign and malignant lesions were processed using xylene (A), 1.7% DWS (B), and RMO (C). Each section was evaluated, scored as 0 (inadequate) and 1 (adequate) by two independent pathologists who were blinded to agent used. Following criteria were considered: nuclear staining, cytoplasmic staining, clarity, crispness, and uniformity. Total score of <2 was graded as inadequate for diagnosis and 3–5 as adequate. Statistical analysis was done using the SPSS software by applying chi-square test. Among three methods, B had the best scores in adequacy for cytoplasmic staining (P = 0.001), clarity (P = 0.004), and crispness (P = 0.003). About 1.7% DWS and RMO were found to be effective methods for deparaffinization and can replace xylene.en_US
dc.identifier.affiliationsDepartment of Pathology, Maulana Azad Medical College, New Delhi, Indiaen_US
dc.identifier.citationYadav S, Mallya V, Khurana N. Xylene-free staining: Is it possible and practical?. Indian Journal of Pathology and Microbiology. 2019 Jun; 62(2): 274-278en_US
dc.identifier.issn0377-4929
dc.identifier.issn0974-5130
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/196332
dc.languageenen_US
dc.publisherIndian Association of Pathologists and Microbiologistsen_US
dc.relation.issuenumber2en_US
dc.relation.volume62en_US
dc.source.urihttps://dx.doi.org/10.4103/IJPM.IJPM_39_18en_US
dc.subjectDishwasheren_US
dc.subjectmineral oilen_US
dc.subjectparaffinen_US
dc.subjectxyleneen_US
dc.titleXylene-free staining: Is it possible and practical?en_US
dc.typeJournal Articleen_US
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