Acetylcholinesterase: Inhibitory activity of some Indonesian vegetables and fraction of selected plants
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Date
2020-01
Journal Title
Journal ISSN
Volume Title
Publisher
Open Science Publishers LLP
Abstract
Dementia is a global health problem that requires severe treatment. People with dementia who consume vegetablesevery day can reduce the risk of dementia. Alzheimer’s is one of the diseases that are characterized by dementia, whichresults in decreased neurotransmitter acetylcholine. This study was to investigate the acetylcholinesterase (AChE)enzyme inhibitory activity of various vegetables that are widely used by Indonesian Sundanese and Javanese ethnics. Inthis study, total phenolic and flavonoid content were analyzed for the determination of active compounds of the extracts.Ethanol extracts of 13 vegetables have been tested for AChE inhibitory activity using Ellman's colorimetric method in96-well plate. Four types of extracts (Cosmos caudatus, Nasturtium officinale, Nothopanax fruticosus, and Ocimumamericanum) had higher total phenolic and flavonoid content than other extracts. Vegetable extracts that had IC50 valuesof less than 1,000 µg/ml were C. caudatus and O. americanum. Both of these extracts were partitioned with n-hexaneand water. The IC50 values of water fraction of C. caudatus and n-hexane fraction of O. americanum were 325.0 ± 18.3and 374.4 ± 42.1 µg/ml, respectively. These results showed that fractions had a potential inhibitor of AChE, and thechemical components (phenolic, flavonoid, and terpenoid) can be isolated to find the active compound.
Description
Keywords
Acetylcholinesterase, flavonoid, phenolic, vegetables.
Citation
Nuria Maulita Cut, Suganda Asep Gana, Sukandar Elin Yulinah, Insanu Muhamad. Acetylcholinesterase: Inhibitory activity of some Indonesian vegetables and fraction of selected plants. Journal of Applied Pharmaceutical Science. 2020 Jan; 2020 Jan: 101-107