Mechanism of cAMP-induced H(+)-efflux of Dictyostelium cells: a role for fatty acids.

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dc.contributor.authorFlaadt, Hen_US
dc.contributor.authorSchaloske, Ren_US
dc.contributor.authorMalchow, Den_US
dc.date.accessioned2000-09-07en_US
dc.date.accessioned2009-06-01T14:55:36Z
dc.date.available2000-09-07en_US
dc.date.available2009-06-01T14:55:36Z
dc.date.issued2000-09-07en_US
dc.description.abstractAggregating Dictyostelium cells release protons when stimulated with cAMP. To find out whether the protons are generated by acidic vesicles or in the cytosol, we permeabilized the cells and found that this did not alter the cAMP-response. Proton efflux in intact cells was inhibited by preincubation with the V-type H(+) ATPase inhibitor concanamycin A and with the plasma membrane H(+) ATPase blocker miconazole. Surprisingly, miconazole also inhibited efflux in permeabilized cells, indicating that this type of H(+) ATPase is present on intracellular vesicles as well. Vesicular acidification was inhibited by miconazole and by concanamycin A, suggesting that the acidic vesicles contain both V-type and P-type H(+) ATPases. Moreover, concanamycin A and miconazole acted in concert, both in intact cells and in vesicles. The mechanism of cAMP-induced Ca2(+)-fluxes involves phospholipase A2 activity. Fatty acids circumvent the plasma membrane and stimulate vesicular Ca2(+)-efflux. Here we show that arachidonic acid elicited H(+)-efflux not only from intact cells but also from acidic vesicles. The target of regulation by arachidonic acid seemed to be the vesicular Ca2(+)-release channel.en_US
dc.description.affiliationFaculty of Biology, University of Konstanz, D-78457 Konstanz, Germany.en_US
dc.identifier.citationFlaadt H, Schaloske R, Malchow D. Mechanism of cAMP-induced H(+)-efflux of Dictyostelium cells: a role for fatty acids. Journal of Biosciences. 2000 Sep; 25(3): 243-52en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/111177
dc.language.isoengen_US
dc.source.urihttps://www.ias.ac.in/jbiosci/index.htmlen_US
dc.subject.mesh4-Chloro-7-nitrobenzofurazan --pharmacologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshAnti-Bacterial Agents --pharmacologyen_US
dc.subject.meshArachidonic Acid --pharmacologyen_US
dc.subject.meshCalcium Signaling --drug effectsen_US
dc.subject.meshCyclic AMP --physiologyen_US
dc.subject.meshDictyostelium --cytologyen_US
dc.subject.meshFatty Acids --physiologyen_US
dc.subject.meshFilipin --pharmacologyen_US
dc.subject.meshHydrogen --metabolismen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshIon Transport --drug effectsen_US
dc.subject.meshMacrolidesen_US
dc.subject.meshMembrane Proteins --antagonists & inhibitorsen_US
dc.subject.meshMiconazole --pharmacologyen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshOrganelles --drug effectsen_US
dc.subject.meshPhospholipases A --physiologyen_US
dc.subject.meshPhospholipases A2en_US
dc.subject.meshProton-Translocating ATPases --antagonists & inhibitorsen_US
dc.subject.meshProtonsen_US
dc.titleMechanism of cAMP-induced H(+)-efflux of Dictyostelium cells: a role for fatty acids.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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