Acridine orange stained blood wet mounts for fluorescent detection of malaria.

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dc.contributor.authorHemvani, Nen_US
dc.contributor.authorChitnis, D Sen_US
dc.contributor.authorDixit, D Sen_US
dc.contributor.authorAsolkar, M Ven_US
dc.date.accessioned1999-04-20en_US
dc.date.accessioned2009-05-29T13:17:20Z
dc.date.available1999-04-20en_US
dc.date.available2009-05-29T13:17:20Z
dc.date.issued1999-04-20en_US
dc.description.abstractThe age old Romanowsky stained thick blood smear examination for malarial parasites may fail to reveal the low parasitaemia. The commercial 'QBC' like acridine orange stained capillary tube preparation has a limitation of precise species identification and the detection of extra-erythrocytic parasites. Hence, the present study was aimed to improve malarial parasite detection by using acridine orange to stain large blood drops in the form of wet coverglass mounts. The acridine orange stained blood wet mounts over 2420 suspected malaria cases from Indore city were examined under fluorescent microscope and the results compared with the Leishman's stained thick blood smears in a blind study. The positivity of malarial parasites reported by the modified acridine orange staining was 248 against 109 by Leishman's stained thick blood smears. The modified acridine orange stained method is simple, instant and more efficient, requires less scanning time and skill, allows scanning of larger blood volume (75 ul) at lower magnification and the morphological details at higher magnification helps to make the precise species identification.en_US
dc.description.affiliationDepartment Pathology, Choithram Hospital, Indore, India.en_US
dc.identifier.citationHemvani N, Chitnis DS, Dixit DS, Asolkar MV. Acridine orange stained blood wet mounts for fluorescent detection of malaria. Indian Journal of Pathology & Microbiology. 1999 Apr; 42(2): 125-8en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/75491
dc.language.isoengen_US
dc.source.urihttps://www.ijpmonline.orgen_US
dc.subject.meshAcridine Orangeen_US
dc.subject.meshAnimalsen_US
dc.subject.meshFluorescent Dyesen_US
dc.subject.meshHumansen_US
dc.subject.meshMalaria --blooden_US
dc.subject.meshMalaria, Falciparum --blooden_US
dc.subject.meshMalaria, Vivax --blooden_US
dc.subject.meshMicroscopy, Fluorescenceen_US
dc.subject.meshPlasmodium falciparum --isolation & purificationen_US
dc.subject.meshPlasmodium vivax --isolation & purificationen_US
dc.subject.meshStaining and Labeling --methodsen_US
dc.titleAcridine orange stained blood wet mounts for fluorescent detection of malaria.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
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