Alpha-thalassemia incidence in southern Thailand by restriction endonuclease analysis of globin DNA from placental blood at Songklanagarind Hospital.

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dc.contributor.authorSriroongrueng, Wen_US
dc.contributor.authorPornpatkul, Men_US
dc.contributor.authorPanich, Ven_US
dc.contributor.authorFucharoen, Sen_US
dc.date.accessioned2009-05-27T15:31:14Z
dc.date.available2009-05-27T15:31:14Z
dc.date.issued1997-03-01en_US
dc.descriptionThe Southeast Asian Journal of Tropical Medicine and Public Health.en_US
dc.description.abstractThe incidence of alpha-thalassemia has been studied previously based on the levels of Hb Barts' in cord blood. This method is an inadequate indicator of alpha-thalassemia. Thus in this study we use DNA analysis to get more accurate data. Hb Barts' was detected in placental blood samples from 15.5% of 375 infants born at Songklanagarind Hospital. The white blood cell DNA of 300 samples was studied for alpha-globin gene deletions by hybridization of DNA fragments digested by the restriction endonuclease Eco RI with specific 32P-labled zeta-globin gene probe. The incidence of alpha-thal 2 and alpha-thal 1 traits were 12.0% and 4.3%, with the gene frequencies 0.0650 and 0.0217 for -alpha/and --/, respectively. The incidence of HB CS trait was 5.8%, with the gene frequency of 0.0292 for alpha cs alpha/. We also found that the incidence of the triplicated zeta and triplicated alpha were 14.7 and 1.0%, with the gene frequencies of 0.0733 and 0.0050 for zeta zeta zeta/and alpha alpha alpha/, respectively. The DNA lesion of alpha-thalassemia in the south is similar to the study of Tanphaichitr et al (1988) in central Thailand. Knowledge of alpha-globin gene deletion would be useful for prenatal diagnosis of Bart's hydrops to prevent toxemia of pregnancy in the south of Thailand.en_US
dc.description.affiliationDepartment of Pathology, Faculty of Medicine, Prince of Songkla University, Thailand.en_US
dc.identifier.citationSriroongrueng W, Pornpatkul M, Panich V, Fucharoen S. Alpha-thalassemia incidence in southern Thailand by restriction endonuclease analysis of globin DNA from placental blood at Songklanagarind Hospital. The Southeast Asian Journal of Tropical Medicine and Public Health. 1997 ; 28 Suppl 3(): 93-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/32986
dc.language.isoengen_US
dc.source.urihttps://www.tm.mahidol.ac.th/seameo/publication.htmen_US
dc.subject.meshDNA --geneticsen_US
dc.subject.meshDNA Restriction Enzymes --diagnostic useen_US
dc.subject.meshFemaleen_US
dc.subject.meshFetal Blood --metabolismen_US
dc.subject.meshGenotypeen_US
dc.subject.meshGlobins --geneticsen_US
dc.subject.meshHemoglobins, Abnormal --geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshIncidenceen_US
dc.subject.meshInfant, Newbornen_US
dc.subject.meshPregnancyen_US
dc.subject.meshThailand --epidemiologyen_US
dc.subject.meshalpha-Thalassemia --diagnosisen_US
dc.titleAlpha-thalassemia incidence in southern Thailand by restriction endonuclease analysis of globin DNA from placental blood at Songklanagarind Hospital.en_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
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