Detection of mutation in isoniazid-resistant Mycobacterium tuberculosis isolates from tuberculosis patients in Belarus.

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dc.contributor.authorBostanabad, S Zen_US
dc.contributor.authorTitov, L Pen_US
dc.contributor.authorBahrmand, Aen_US
dc.contributor.authorNojoumi, S Aen_US
dc.date.accessioned2008-04-01en_US
dc.date.accessioned2009-05-28T09:12:42Z
dc.date.available2008-04-01en_US
dc.date.available2009-05-28T09:12:42Z
dc.date.issued2008-04-01en_US
dc.description.abstractThe aim of this study was to investigate the frequency, location and type of katG mutations in Mycobacterium tuberculosis strains isolated from patients in Belarus. Forty two isoniazid-resistant isolates were identified from sputum of 163 patients with active pulmonary tuberculosis. Drug susceptibility testing was determined by using CDC standard conventional proportional method and BACTEC system. Standard PCR method for detection of isoniazid resistance associated mutations was performed by katG gene amplification and DNA sequencing. Most mutations were found in katG gene codons 315, 316 and 309. Four types of mutations were identified in codon 315: AGC-->ACC (n=36) 85%, AGC-->AGG (n=1) 2.3%, AGC-->AAC (n=2) 4.7%, AGC-->GGC (n=1) 2.3%. One type of mutation was found in codon 316: GGC-->AGC (n=18) 41.4%, four types of mutations were detected in codon 309: GGT-->GGT (n=7) 16.1%, GGT-->GCT (n=4) 9.2%, GGT-->GTC (n=3)6.9%, GGT-->GGG (n=1) 2.7%. The highest frequency of mutations sharing between primary and secondary infections was found in codon 315.en_US
dc.description.affiliationBelarusian Research Institute for Epidemiology and Microbiology, Filimonova 23, Minsk, Belarus. saeed1976@mail.ruen_US
dc.identifier.citationBostanabad SZ, Titov LP, Bahrmand A, Nojoumi SA. Detection of mutation in isoniazid-resistant Mycobacterium tuberculosis isolates from tuberculosis patients in Belarus. Indian Journal of Medical Microbiology. 2008 Apr-Jun; 26(2): 143-7en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/53593
dc.language.isoengen_US
dc.source.urihttps://www.ijmm.orgen_US
dc.subject.meshAmino Acid Substitution --geneticsen_US
dc.subject.meshAntitubercular Agents --pharmacologyen_US
dc.subject.meshBacterial Proteins --geneticsen_US
dc.subject.meshByelarusen_US
dc.subject.meshCatalase --geneticsen_US
dc.subject.meshDNA, Bacterial --chemistryen_US
dc.subject.meshDrug Resistance, Bacterial --geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshIsoniazid --pharmacologyen_US
dc.subject.meshMicrobial Sensitivity Testsen_US
dc.subject.meshMutation, Missenseen_US
dc.subject.meshMycobacterium tuberculosis --drug effectsen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshSequence Analysis, DNAen_US
dc.subject.meshSputum --microbiologyen_US
dc.subject.meshTuberculosis --microbiologyen_US
dc.titleDetection of mutation in isoniazid-resistant Mycobacterium tuberculosis isolates from tuberculosis patients in Belarus.en_US
dc.typeJournal Articleen_US
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