Effect of ischemia/reperfusion on intestinal brush border membrane lipid composition, fluidity and enzyme activities.

Simple item page
dc.contributor.authorAhamed Ibrahim, Sen_US
dc.contributor.authorBasker, Len_US
dc.contributor.authorBalasubramanian, K Aen_US
dc.date.accessioned1996-02-01en_US
dc.date.accessioned2009-05-27T09:58:51Z
dc.date.available1996-02-01en_US
dc.date.available2009-05-27T09:58:51Z
dc.date.issued1996-02-01en_US
dc.description.abstractOxygen-derived free radicals are known to be generated during ischemia/reperfusion injury and biomembranes are the prime target of these active species. In order to study the effect of in vivo generated free radicals on intestinal mucosal membrane, brush border membranes (BBM) were isolated from rat small intestine after subjecting to ischemia (I) and ischemia/reperfusion (I/R) injury and their lipid composition and marker enzyme activity were compared with BBM prepared from control animals. No significant alteration in the lipid composition of BBM was observed after I or I/R as compared to control. Membrane fluidity measurements showed that I/R increased the fluidity of BBM. Activity of alkaline phosphatase, one of the marker enzymes for BBM was reduced by I or I/R whereas activity of another BBM enzyme, sucrase was not altered. The decrease in alkaline phosphatase activity was more after reperfusion. In vitro fluidization of BBM using benzyl alcohol indicated that the inactivation of alkaline phosphatase was not due to change in fluidity. These results suggest that free radicals generated during I/R inactivate BBM alkaline phosphatase partially without altering the membrane lipid composition.en_US
dc.description.affiliationDepartment of Gastrointestinal Sciences, Christian Medical College & Hospital, Vellore, India.en_US
dc.identifier.citationAhamed Ibrahim S, Basker L, Balasubramanian KA. Effect of ischemia/reperfusion on intestinal brush border membrane lipid composition, fluidity and enzyme activities. Indian Journal of Biochemistry & Biophysics. 1996 Feb; 33(1): 53-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/29127
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAlkaline Phosphatase --metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshIntestinal Mucosa --blood supplyen_US
dc.subject.meshIntestines --blood supplyen_US
dc.subject.meshIschemia --metabolismen_US
dc.subject.meshMembrane Fluidityen_US
dc.subject.meshMembrane Lipids --metabolismen_US
dc.subject.meshMicrovilli --metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshReperfusionen_US
dc.subject.meshSucrase --metabolismen_US
dc.titleEffect of ischemia/reperfusion on intestinal brush border membrane lipid composition, fluidity and enzyme activities.en_US
dc.typeComparative Studyen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
Files
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.79 KB
Format:
Plain Text
Description:
Download
Collections
Indian Journal of Biochemistry & Biophysics