Adaptation of microsomal glutathione transferase 1 in PC12 cells with modified PMCA isoforms composition.

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dc.contributor.authorBoczek, Tomasz
dc.contributor.authorKozaczuk, Anna
dc.contributor.authorTaha, Joanna
dc.contributor.authorFerenc, Bozena
dc.contributor.authorZylinska, Ludmila
dc.date.accessioned2011-11-14T10:01:41Z
dc.date.available2011-11-14T10:01:41Z
dc.date.issued2010-10
dc.description.abstractMicrosomal glutathione transferase 1 (MGST1) is an integral homo-trimeric membrane protein with transferase and peroxidase activities. With glutathione as a co-substrate, it scavenges toxic compounds and may exert anti-apoptotic effect. We examined the effect of suppression of plasma membrane Ca2+-ATPase isoforms — PMCA2 or PMCA3 on MGST1 in PC12 cells. GSH level was significantly higher in PMCA2-reduced line, but similar GSSG/GSH ratios in all cell lines suggested an efficient protection or absence of oxidative stress. The ATP concentration decreased in both modified lines, although in PMCA2-suppressed cells the decrease was higher. Total GSTs activity in postmitochondrial fraction increased by 30% in the cells with reduced PMCA3. After treatment with MGST1 activator N-ethylmaleimide (NEM), the activity increased in both transfected lines by 30-40%. Real-time PCR also showed a higher mRNA expression of MGST1 in these lines. Staining with antibody recognizing all cytosolic and membrane-bound GSTs revealed the difference in oligomeric forms of GSTs, and specific anti-MGST1 antibody showed the presence of MGST1 hexamers in the transfected cells. Formation of similar hexamers was detected in the control line after treatment with peroxynitrite. Modification of MGST1 under reduced PMCAs amount may represent an adaptive mechanism that offers protection against the cytotoxicity mediated by increased Ca2+.en_US
dc.identifier.citationBoczek Tomasz, Kozaczuk Anna, Taha Joanna, Ferenc Bozena, Zylinska Ludmila. Adaptation of microsomal glutathione transferase 1 in PC12 cells with modified PMCA isoforms composition. Indian Journal of Biochemistry & Biophysics. 2010 Oct; 47(5): 265-271.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/135275
dc.language.isoenen_US
dc.source.urihttps://nopr.niscair.res.in/bitstream/123456789/10519/1/IJBB%2047%285%29%20265-271.pdfen_US
dc.subjectPlasma membrane Ca2+-ATPaseen_US
dc.subjectPC12 cellen_US
dc.subjectGlutathioneen_US
dc.subjectGlutathione transferasesen_US
dc.subjectPeroxynitriteen_US
dc.subject.meshAdaptation, Physiological --physiology
dc.subject.meshAnimals
dc.subject.meshEnzyme Activation
dc.subject.meshGlutathione Transferase --metabolism
dc.subject.meshMicrosomes --enzymology
dc.subject.meshPC12 Cells
dc.subject.meshPlasma Membrane Calcium-Transporting ATPases --metabolism
dc.subject.meshRats
dc.titleAdaptation of microsomal glutathione transferase 1 in PC12 cells with modified PMCA isoforms composition.en_US
dc.typeArticleen_US
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