Evolutionary divergence of function and expression of laccase genes in plants

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dc.contributor.authorLiu, Mingyueen_US
dc.contributor.authorDong, Huien_US
dc.contributor.authorWang, Meien_US
dc.contributor.authorLiu, Qingpoen_US
dc.date.accessioned2020-11-18T10:21:09Z
dc.date.available2020-11-18T10:21:09Z
dc.date.issued2020-03
dc.description.abstractLaccases (LACs) are versatile enzymes that catalyze oxidation of a wide range of substrates, thereby functioning in regulation of plant developmental processes and stress responses. However, with a few exceptions, the function of most LACs remains unclear in plants. In this study, we newly identified 4, 12, 22, 26, 27, 28 and 49 LAC genes for Physcomitrella patens, Amborella trichopoda, Zea mays, Ricinus communis, Vitis vinifera, Triticum aestivum and Glycine max, on the basis of exhaustive homologous sequence searches. In these plants, LACs differ greatly in sequence length and physical properties, such as molecular weight and theoretical isoelectric point (pI), but majority of them contain a signal peptide at their N-terminus. The originality of LACs could be traced back to as early as the emergence of moss. Plant LACs are clearly divided into seven distinct classes, where six ancient LACs should be present prior to the divergence of gymnosperms and angiosperms. Functional divergence analysis reveal that functional differentiation should occur among different groups of LACs because of altered selective constraints working on some critical amino acid sites (CAASs) within conserved laccase domains during evolution. Soybean and maize LACs have significantly different exon frequency (6.08 vs 4.82), and they are unevenly distributed and tend to form gene clusters on some chromosomes. Further analysis shows that the expansion of LAC gene family would be due to extensive tandem and chromosomal segmental duplications in the two plant species. Interestingly, *81.6% and 36.4% of soybean and maize LACs are potential targets of miRNAs, such as miR397a/b, miR408d, or miR528a/b etc. Both soybean and maize LACs are tissuespecifically and developmental-specifically expressed, and are in response to different external abiotic and biotic stressors. These results suggest a diversity of functions of plant LAC genes, which will broaden our understanding and lay solid foundation for further investigating their biological functions in plants.en_US
dc.identifier.affiliationsThe State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Lin’an, Hangzhou 311300, People’s Republic of Chinaen_US
dc.identifier.affiliationsThe Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agriculture and Food Sciences, Zhejiang A&F University, Lin’an, Hangzhou 311300, People’s Republic of Chinaen_US
dc.identifier.affiliationsThe Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agriculture and Food Sciences, Zhejiang A&F University, Lin’an, Hangzhou 311300, People’s Republic of Chinaen_US
dc.identifier.affiliationsThe Key Laboratory for Quality Improvement of Agricultural Products of Zhejiang Province, College of Agriculture and Food Sciences, Zhejiang A&F University, Lin’an, Hangzhou 311300, People’s Republic of Chinaen_US
dc.identifier.citationLiu Mingyue, Dong Hui, Wang Mei, Liu Qingpo. Evolutionary divergence of function and expression of laccase genes in plants. Journal of Genetics. 2020 Mar; 99: 1-16en_US
dc.identifier.issn0022-1333
dc.identifier.issn0973-7731
dc.identifier.placeIndiaen_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/215540
dc.languageenen_US
dc.publisherIndian Academy of Sciencesen_US
dc.relation.volume99en_US
dc.source.urihttps://doi.org/10.1007/s12041-020-1184-0en_US
dc.subjectplanten_US
dc.subjectlaccaseen_US
dc.subjectphylogenyen_US
dc.subjectfunctional divergenceen_US
dc.subjectgene expression.en_US
dc.titleEvolutionary divergence of function and expression of laccase genes in plantsen_US
dc.typeJournal Articleen_US
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