2, 3, 7, 8-tetrachloro-dibenzo-p-dioxin induced testicular toxicity in rats and the protective effect of quercetin: Biochemical, histopathological and immunohistochemical studies.
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Date
2015-01
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Abstract
Humans and animals are most sensitive to toxicant exposure during development. Dioxin, as an endocrine
disruptor, is known to impair testicular functions and fertility. The present study was carried out to investigate
the effects of quercetin on TCDD-induced toxicity in the testicular tissue of rats. Forty male albino rats were
randomly divided into four groups (n = 10/group). Group I represent the control group; Group II administrated
TCDD (27.5 μg/kg) via gavage for four week; Group III received quercetin (20 mg/kg bw.) Via gavage before
TCDD administration; Group IV received quercetin alone (20 mg/kg bw). Biochemical markers included
levels of testicular malondialdehyde formation and reduced glutathione as well as monitoring the activities of
testicular superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase were studied. Also,
serum hormonal profiles of luteinizing hormone and testosterone were reported. Our results show that
administration of TCDD induces testicular damage concerning oxidative stress parameters, serum hormone
level and sperm parameters. In addition, the microscopic structures of the testis, including histological and
immunohistochemical studies were evaluated. Exposure to TCDD induces histopathological changes in rats
testis including degeneration of seminiferous tubules, tubular necrosis, intratubular vacuolization, widened
lumen and deshaped germ cells. Marked increase of apoptotic activity was observed. Also, our results clearly
demonstrate the ameliorative potential of quercetin in dioxin induced testicular damage.
Description
Keywords
TCDD, Quercetin, Testis, Oxidative stress
Citation
El-Gerbed Mohamed S A, El-Saad Ahmed M Abu, Haussein Abdullah Bedeer. 2, 3, 7, 8-tetrachloro-dibenzo-p-dioxin induced testicular toxicity in rats and the protective effect of quercetin: Biochemical, histopathological and immunohistochemical studies. Journal of Applied Pharmaceutical Science. 2015 Jan; 5(1): 99-109.