Rat brain membrane-bound delta opioid receptor: loss and reactivation of binding on dialysis and aging at low temperature.

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dc.contributor.authorChristadoss, Een_US
dc.contributor.authorOommen, Aen_US
dc.date.accessioned2001-06-06en_US
dc.date.accessioned2009-05-27T09:07:11Z
dc.date.available2001-06-06en_US
dc.date.available2009-05-27T09:07:11Z
dc.date.issued2001-06-06en_US
dc.description.abstractA change in the environment of rat brain membranes by dialysis from phosphate buffered saline (PBS) to 10 mM potassium phosphate (pH 7.2) led to a 35% loss in delta opioid receptor binding, while alteration of membrane structure on freezing at -20 degrees C for 55 days led to 85% loss of receptor binding. The dialysate, 200 mM KCI and NaCl restored receptor binding lost on dialysis. This K+ and Na+ restabilization of the receptor can be through cation-pi bonding, interactions that are suited to the lipid bilayer. In membranes stored at -20 degrees C, the loss of binding is attributed to increased membrane fluidity by phospholipase A2 action on membrane phospholipids, resulting in an increase of free fatty acids. K+ but not Na+ restabilization of these membrane receptors may be due to the ability of K+ to decrease membrane fluidity.en_US
dc.description.affiliationNeurochemistry Laboratory, Department of Neurological Sciences, CMC Hospital, Vellore, TN, India.en_US
dc.identifier.citationChristadoss E, Oommen A. Rat brain membrane-bound delta opioid receptor: loss and reactivation of binding on dialysis and aging at low temperature. Indian Journal of Biochemistry & Biophysics. 2001 Jun; 38(3): 166-9en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/26510
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBrain --metabolismen_US
dc.subject.meshCell Membrane --metabolismen_US
dc.subject.meshDialysisen_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshPhospholipases A --metabolismen_US
dc.subject.meshPhospholipases A2en_US
dc.subject.meshPhospholipids --metabolismen_US
dc.subject.meshPotassium --metabolismen_US
dc.subject.meshProtein Bindingen_US
dc.subject.meshRatsen_US
dc.subject.meshReceptors, Opioid, delta --metabolismen_US
dc.subject.meshSodium --metabolismen_US
dc.subject.meshTemperatureen_US
dc.subject.meshTime Factorsen_US
dc.titleRat brain membrane-bound delta opioid receptor: loss and reactivation of binding on dialysis and aging at low temperature.en_US
dc.typeJournal Articleen_US
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