Refined procedures for accurate determination of solution structures of nucleic acids by two dimensional nuclear magnetic resonance spectroscopy.
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Date
1988-03
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Abstract
New procedures have been described for accurate determination of solution
structures of nucleic acids. These are two fold; new two dimensional nuclear magnetic
resonance techniques and better approaches for interpretation of nuclear magnetic
resonance data for structure determination purposes. The significant development in two
dimensional nuclear magnetic resonance techniques for this purpose are ω1 -scaling and
recording of pure phase spectra. Use of ω1-scaled correlated and nuclear Overhauser effect
spectra for estimation of interproton distances and 1H-1H coupling constants has been
described. Computer simulation procedures for exact determination of structure have been
described. Experimental spectra demonstrating the application of new procedures have been
presented.
Description
Keywords
Nucleic acids, ω1-scaling, 2D NMR. computer simulation, inter proton distances
Citation
Hosur R V, Chary K V R, Sheth A, Govil Girjesh, Miles H T. Refined procedures for accurate determination of solution structures of nucleic acids by two dimensional nuclear magnetic resonance spectroscopy. Journal of Biosciences. 1988 Mar; 13(1): 71-86.