Establishment of real-time polymerase chain reaction-based assay for quantitation of Epstein-Barr virus DNA in healthy donors and in patients with EBV associated lymphoma.

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dc.contributor.authorIncomserb, Patchaen_US
dc.contributor.authorBhattarakosol, Parvapanen_US
dc.contributor.authorKulwichit, Wanlaen_US
dc.contributor.authorChantratita, Wasunen_US
dc.contributor.authorHansasuta, Pokrathen_US
dc.date.accessioned2009-05-27T19:20:14Z
dc.date.available2009-05-27T19:20:14Z
dc.date.issued2005-09-21en_US
dc.descriptionChotmaihet Thangphaet.en_US
dc.description.abstractEpstein-Barr virus (EBV) is associated with several malignancies including nasopharyngeal carcinoma and lymphoma in immunocompromised patients. Quantitative monitoring of EBV DNA in these patients has recently become essential for management of the disease. In the present study the authors developed a rapid and reliable real-time PCR to quantify the EBV DNA in peripheral blood mononuclear cell (PBMC) using hybridization probe technique. The real-time primers and probes in this real-time PCR system were designed based on EBNA-1 sequence. The newly-established real-time PCR demonstrated its high sensitivity (as few as 10 copies of EBV could be detected) and specificity. The intra- and inter-assay variations of the assay were shown to be within a 0.5-log10-difference range. A total of 2 EBV-seronegative, 14 EBV-seropositive healthy donors and 4 patients with PCNSL were enrolled into the study. Our results revealed the median of EBV-DNA in lymphoma patients (7886 copies/10(6) PBMC or 15,150 copies /microg DNA) was higher than that of healthy donors (<10 copies/l0(6) PBMC or <10 copies/microg DNA) with statistic significance (P < 0.01). Assessment of this assay in larger number of donors and patients will provide clinical cut-off values which are essential for monitoring and diagnosis of EBV-associated diseases.en_US
dc.description.affiliationInter-department of Medical Microbiology, Faculty of Graduate School, Chulalongkorn University, Bangkok, Thailand.en_US
dc.identifier.citationIncomserb P, Bhattarakosol P, Kulwichit W, Chantratita W, Hansasuta P. Establishment of real-time polymerase chain reaction-based assay for quantitation of Epstein-Barr virus DNA in healthy donors and in patients with EBV associated lymphoma. Journal of the Medical Association of Thailand. 2005 Sep; 88 Suppl 4(): S280-6en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/40930
dc.language.isoengen_US
dc.source.urihttps://www.mat.or.th/journal/all.phpen_US
dc.subject.meshAdulten_US
dc.subject.meshBlood Donorsen_US
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshComputer Systemsen_US
dc.subject.meshDNA, Viral --analysisen_US
dc.subject.meshEpstein-Barr Virus Infections --complicationsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHerpesvirus 4, Human --isolation & purificationen_US
dc.subject.meshHumansen_US
dc.subject.meshLymphoma, T-Cell --physiopathologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction --methodsen_US
dc.subject.meshTime Factorsen_US
dc.titleEstablishment of real-time polymerase chain reaction-based assay for quantitation of Epstein-Barr virus DNA in healthy donors and in patients with EBV associated lymphoma.en_US
dc.typeEvaluation Studiesen_US
dc.typeJournal Articleen_US
dc.typeResearch Support, Non-U.S. Gov'ten_US
dc.typeValidation Studiesen_US
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