SXT constin among Vibrio cholerae isolates from a tertiary care hospital.

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dc.contributor.authorPande, Kavita
dc.contributor.authorMendiratta, Deepak Kumar
dc.contributor.authorVijayashri, Deotale
dc.contributor.authorThamke, Dipak C
dc.contributor.authorNarang, Pratibha
dc.date.accessioned2012-06-13T04:08:52Z
dc.date.available2012-06-13T04:08:52Z
dc.date.issued2012-03
dc.description.abstractBackground & objectives: The SXT element, also known as ‘constin’ (conjugable, self transmissible, integrating element) is an integrating conjugative element (ICE) in Vibrio cholerae discovered in the chromosome of epidemic V. cholerae O139 strain MO10 (SXTMO10) which arose in late 1992 in Chennai, India. SXT related ICEs have become widespread and currently, most if not all Asian V. cholerae clinical isolates contain SXT related ICEs. The present study attempts to determine the presence of SXT Int gene in V. cholerae recovered between 2005 to 2007 in a tertiary care hospital, demonstrate its conjugal nature and also detect co-presence and co-transfer of plasmids in representative isolates. Methods: This prospective study was done on 116 V. cholerae isolates [114- O1 (107 ogawa and 7 inaba) and 2 - Non O1 Non O139 V. cholerae] from watery stools between 2005 to 2007 recovered from equal number of patients. PCR was carried out using SXT Int specific primers that produced a 592 bp internal fragment of SXT element, and rifampicin resistant strain of E.coli K-12 was used as recipient in conjugation experiments to study transfer of SXT, as also co-transfer of resistance to tetracycline, erythromycin, and nalidixic acid. Antibiotic susceptibility was performed against various antibiotics. Results: Of the 116 isolates, 110 (94.8%) were positive for SXT element by PCR. It was demonstrated in 94.7 per cent of the O1, and 100 per cent of non O1 non O139 V. cholerae. All 2005 isolates, 25 per cent of 2006 isolates and 96.6 per cent of 2007 isolates were positive for SXT. Thirty two drug resistance patterns were observed and the 2007 isolates showed resistance to as many as eight antibiotics. The resistance of SXT positive isolates was higher than those of SXT negative and the typical drug resistance pattern corresponding to SXTET and SXTMO10 was shown by only one V. cholerae O1 isolate. Successful conjugal transfer of SXT was seen in 31 (88.6%) of the 35 isolates studied without any co-transfer while, presence of plasmids was observed in two of the 31 donor V. cholerae studied. Interpretation & Conclusions: The demonstration of SXT element and its successful horizontal transfer in V. cholerae isolates studied emphasizes the need for its detection to monitor antibiotic resistance and dissemination in V. cholerae.en_US
dc.identifier.citationPande Kavita, Mendiratta Deepak Kumar, Vijayashri Deotale, Thamke Dipak C, Narang Pratibha. SXT constin among Vibrio cholerae isolates from a tertiary care hospital. Indian Journal of Medical Research. 2012 Mar; 135(3): 346-350.en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/138999
dc.language.isoenen_US
dc.source.urihttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3361871/en_US
dc.subjectConjugal transferen_US
dc.subjectconstinen_US
dc.subjectdrug resistanceen_US
dc.subjectSXT ICEen_US
dc.subjectV choleraeen_US
dc.subject.meshBacterial Proteins --genetics
dc.subject.meshBacterial Proteins --metabolism
dc.subject.meshCholera --microbiology
dc.subject.meshDNA Transposable Elements
dc.subject.meshHumans
dc.subject.meshInterspersed Repetitive Sequences
dc.subject.meshProspective Studies
dc.subject.meshVibrio cholerae --drug effects
dc.subject.meshVibrio cholerae --genetics
dc.subject.meshVibrio cholerae --isolation & purification
dc.subject.meshVibrio cholerae --metabolism
dc.subject.otherDrug Resistance, Bacterial
dc.titleSXT constin among Vibrio cholerae isolates from a tertiary care hospital.en_US
dc.typeArticleen_US
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