Effect of light on nucleotide modifications in the transfer RNA of cucumber cotyledons.
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Date
1988-12
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Abstract
The effect of light on nucleotide modifications in the tRNA of cucumber
(Cucumis sativus L. var. Guntur) cotyledons was studied by chromatographic, electrophoretic
and immunological methods. The tRNA from light-grown tissue showed the
absence of 2-methylguanosine and a decrease in the relative proportions of ribothymidine
and cytokinin-active ribonucleosides when compared to those produced from dark-grown
tissue. On the other hand, a significant amount of one species of 2'-O-methyldinucleotide
was observed in the tRNA of light-grown tissue which was not detected in the dark-grown
tissue. Also, tRNA from light-grown tissue had higher levels of another species of 2'-Omethyldinucleotide.
The results showed no difference in the amounts of other modified
nucleosides in tRNA between tissues grown under the two conditions. 2'-O-Methyl-lmethyladenosine,
a nucleotide modified both in the base and the ribose, apparently specific
to plant tRNAs, has been found to be present in the RNA of both light- and dark-grown
tissues. These results on the variation in modified nucleotides suggest that light has some
role in nucleotide modification and, consequently, in cellular functions.
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Keywords
tRNA, light, modified nucleotides, Cucumis sativus
Citation
Raju M Putta, Jayabaskaran C. Effect of light on nucleotide modifications in the transfer RNA of cucumber cotyledons. Journal of Biosciences. 1988 Dec; 13(4): 367-378.