Identification of a non-microsomal cinnamic acid 4-hydroxylase from potato tuber (S. tuberosum) and its partial purification.

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dc.contributor.authorDeshpande, A Sen_US
dc.contributor.authorSurendranathan, K Ken_US
dc.contributor.authorNair, P Men_US
dc.date.accessioned1992-10-01en_US
dc.date.accessioned2009-05-27T09:31:54Z
dc.date.available1992-10-01en_US
dc.date.available2009-05-27T09:31:54Z
dc.date.issued1992-10-01en_US
dc.description.abstractThe cytoplasmic localisation of cinnamic acid 4-hydroxylase (CA4H) has been shown by isolation and subcellular fractionation of the enzyme in Hepes buffer. The enzyme was purified by ammonium sulphate fractionation followed by AcA-34 molecular sieve chromatography. The enzyme existed as a high molecular mass which dissociated to a lower form on dilution on the column. The pH optimum, sulphydryl requirement, molecular and preliminary kinetic characteristics were investigated.en_US
dc.description.affiliationFood Technology and Enzyme Engineering Division, Bhabha Atomic Research Centre, Bombay.en_US
dc.identifier.citationDeshpande AS, Surendranathan KK, Nair PM. Identification of a non-microsomal cinnamic acid 4-hydroxylase from potato tuber (S. tuberosum) and its partial purification. Indian Journal of Biochemistry & Biophysics. 1992 Oct; 29(5): 418-24en_US
dc.identifier.urihttps://imsear.searo.who.int/handle/123456789/27787
dc.language.isoengen_US
dc.source.urihttps://https://www.niscair.res.in/ScienceCommunication/ResearchJournals/rejour/ijbb/ijbb0.aspen_US
dc.subject.meshCations, Divalenten_US
dc.subject.meshCell Fractionationen_US
dc.subject.meshCytochrome P-450 Enzyme System --isolation & purificationen_US
dc.subject.meshHydrogen-Ion Concentrationen_US
dc.subject.meshKineticsen_US
dc.subject.meshMicrosomes --enzymologyen_US
dc.subject.meshMixed Function Oxygenases --isolation & purificationen_US
dc.subject.meshSolanum tuberosum --enzymologyen_US
dc.subject.meshSubcellular Fractions --enzymologyen_US
dc.subject.meshTrans-Cinnamate 4-Monooxygenaseen_US
dc.titleIdentification of a non-microsomal cinnamic acid 4-hydroxylase from potato tuber (S. tuberosum) and its partial purification.en_US
dc.typeJournal Articleen_US
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